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Bone Marrow Stromal cell-derived new growth factor for controlling the growth and apoptosis of myeloma cell

Research Project

Project/Area Number 06670507
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionKanazawa Medical University

Principal Investigator

SHIMIZU Shiro  Kanazawa Medical University Associate Professor, 医学部, 助教授 (50097432)

Co-Investigator(Kenkyū-buntansha) MASAKI Yasufumi  Kanazawa Medical University Assistant, 医学部, 助手 (40238895)
ARAI Toshihide  Kanazawa Medical University Assistant, 医学部, 助手 (30193048)
TAKESHITA Shouichi  Kanazawa Medical University Assistant, 医学部, 助手 (50247441)
FUKUTOKU Masaaki  Kanazawa Medical University Assistant, 医学部, 助手 (20218944)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMultiple Myeloma / Growth Factor / Stroma Cell / Apoptosis / multiple myeloma / Growth Factor
Research Abstract

From previous experiments, it was expected that an unknown bone marrow stromal cell derived myeloma cell growth factor playd an important role in the growth of myeloma cells. We have analyzed the growth factor activity to understand the mechanisms of myeloma cell growth.
Following data were obtained : 1) Three bone marrow stromal cell dependent myeloma cell lines, ILKM10, ILKM12 and ILKM13, were established. 2) All three cell lines grew absolutely dependently on bone marrow stromal cells. They did not proliferate in response to known cytokines including IL-6 or gp-130-related cytokines, but proliferate in response to bone marrow stromal cell culture supernatants. 3) The growth factor activity included in the culture supernatant was semipurified utilizing an ion exchange column chromatography and an anti-IL6 antiboy affinity column, and the myeloma growth factor activity was separated from IL-6. Data confirmed that at least another unknown factor playd an important role in the growth of myeloma cells.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 清水史郎: "造血器腫瘍と接着分子" 血液腫瘍科. 28. 152-163 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 清水史郎: "骨髄細胞増殖機構" 日本臨床. 53. 557-563 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 清水史郎: "免疫グロブリン異常の基礎的事項-多発性骨髄腫細胞増殖機構" 日本内科学会雑誌. 84. 1055-1060 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 清水史郎他: "骨髄腫細胞の増殖とアポトーシス" 臨床科学. 32. 711-716 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 福徳雅章、清水史郎: "gp130関連因子と骨髄腫細胞増殖機構" 臨床血液. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiro Shimizu: "Expression of adhesion molecules in hematological malignancies" Hematology & Oncology. 28. 152-163 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiro Shimizu: "The mechanisms of myeloma cell growth" Jpn.J.Clin.Med.53. 557-563 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiro Shimizu: "The mechanisms of myeloma cell growth" Jpn.J.Internal Med.84. 1055-1060 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiro Shimizu, et al.: "The growth and apoptosis of myeloma cell growth" J.Clin.Scien.32. 711-716 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaaki Fukutoku & Shiro Shimizu: "gp-130-related cytokines on the growth of myeloma cells" Jpn.J.Clin.Haemaol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 清水史郎: "I.免疫グロブリン異常の基礎的事項3.多発性骨髄腫細胞増殖機構" 日本内科学会雑誌. 84. 15-20 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 清水史郎: "骨髄腫細胞増殖機構" 日本臨床. 53. 557-563 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ueno, Y. Shimizu, et al.: "Non-Hodgkin′s Lymphoma and Polarthritis" British Journal of Rheumatology. 34. 293-293 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 清水史郎 他: "骨髄腫細胞の増殖とアポトーシス" 臨床科学. 32(予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 清水史郎: "骨髄腫細胞増殖機構" 日本臨床. 53. 557-563 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 清水史郎: "造血器腫瘍と接着分子" 血液-腫瘍科. 28. 152-163 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 正木康史、清水史郎他: "VEP-IFN-αが奏効した非分泌型plasma cell leukemia(BJ-k)の一例" 臨床血液. 35. 562-567 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] P.Yoshioka,S.Shimizu,ct al.: "Interleukin-6 and soluble interleukin-6 receptor in synovial fluid of patients with rheumatoid arthritis" Japanese Journal of Rheumatology. 5. 175-182 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Sugai,S.Shimizu,et al: "Rearrangement of thre Rheumatoid Factor-Related Germline Gene Vg and bcl-2 Expression in Lymphoproliferative Disorders in Patients with Sjogren′s Syndrome" Clinical Immumology & Immunopathology. 72. 181-186 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Yamazaki,S.Shimizu,et al: "Leukemic Meningitis in a Patient with Splenic Lymphoma with Villous Lymphocytes(SLVL)" CANCER. 74. 61-65 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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