• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

NEW METHOD FOR QUANTITATIVE MEASUREMENT OF HELICOBACTER PYLORI IN GASTRIC MUCUS AND GASTRIC MUCOSA.

Research Project

Project/Area Number 06670535
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionHAMAMATSU UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

KANEKO Eizo  HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (10010414)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsHELICOBACTER PYRORI / COMPETITIVE-PCR / PEPTIC ULCER / ヘリコパクターピロリ / competitive PCR法 / Helicobacter pylori / プロトンポンプインヒビター / H.pyloriの除菌 / pepsinogen I / II
Research Abstract

Objectives : Helicobacter pylori (Hp) is closely related to upper gastrointestinal diseases. Establishment of a precise diagnostic device for Hp is of great value. The aim of this study was establishment of a method for quantitatively detecting the bacteria. Methods : We developed a new methods for quantitative detection of Hp by competitive polymerase chain reaction (cPCR) using various known amounts of synthetic DNA fragments containing the same primer template sequences as the target competing for primer binding and amplification. The quantity of Hp in gastric mucus and gastric tissue was measured by this method. The results obtained with this method in gastric mucus were compared with results of histology, rapid urease test (RUT), bacterial culture, and ^<13>C-urea breath test (^<13>C-UBT). The distribution of Hp in the gastric mucosa was also studied quantitatively in the conection with gastric function. Results : Relatively large quantity (10^5-10^9/mucus 100mul) of Hp were found … More by cPCR in gastric mucus when results of histology, RUT or bacterial culture were positive. Even when results of histology, RUT or bacterial culture for Hp were negative, a considerable quantity of Hp in gastric mucus (10^5-10^<8/> mucus 100mul) was often found by cPCR.The quantity of Hp in gastric mucus is significantly correlated with results of ^<13>C-UBT (p<0.0005). We also confirmed that this method was able to distinguish the true negative from the false negative due to incomplete PCR,and the true positive from the false positive due to contamination. As for the distribution of Hp, quantity of Hp in corpus was larger than that in antrum in patients with GU.Quantity of Hp in corpus was smaller than that of antrum in patients with DU.The pepsinogen I/II ratio in the serum significantly increases by eradication of Hp and this change of the ratio was confirmed to be an useful index for the eradicaiton. Conclusions : These results suggest that the cPCR method is very useful for precise and quantitative evaluation of Hp infection in the stomach. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Furuta T: "Effects of Lansoprazole with or without amoxiuillin on ulcer healing:relation to eradicatian" J cein Gartvuenterol. 20(suppl). 109-111 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito G: "Factors affecting quality of ulcer healing after Lansoprazole treafmout." J cein gartvuenterol. 20(suppl). 62-66 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 古田隆久: "血清ペプシノーゲン・ガストリン値の変化からみたHelicobafarpylonの除菌判定" Prog in Medicine. 15. 1862-1868 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T: "Helicolsacter pylori-pvesont stuhus in Japan and a newer diagnortic medhod applying compejjve-PCR" Chinese J Gartneufoul.Hepadol.5,(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T: "Quantitative study of Helico Gaifer pyleri in gartvic wucus by competitive PCR using sythefic DNA fagweits." J Clin Miciodiology. (submi tted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T: "Pevcentage changes in serum pepiinogeus au usejul as in dexes of eradication of Helicobafer pylni" Amev J Gastroeufevol. (submit ted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T et al: "Effects of Lansoprazole with or without amoxicillin on ulcer healing : relation to eradication" J Clin Gastroenterol. 20 (Suppl). 107-111 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito G et al: "Factors affecting quality of ulcer healing after Lansoprazole treatment" Clin Gastroenterl. 20 (Suppl). 62-66 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T et al: "Helicobacter pylori-present status in Japan and a newer daignostic method applying competitive PCR" Chines J Gastroenterol Hepatol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T et al: "Quantitative study of Helicobacter pylori in gastric mucosa by competitive PCR using synthetic DNA fragments" J Clin Microbiol. (submited).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T et al: "Percentage changes in serum pepsinogen are useful as indexes of eradication of Helicobacter pylori" Amer J Gastroenterl. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Furuta T: "Helicoba cfer pylori-preseut studus in Japan and a newer diagnasfic method applying compatitive PCR" Chinese J. Gastroeufeol. Hepafol.5(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 古田隆久: "血清ペプシトゲン・ガストリン値の変化からみたHelico Eacter pylorin除菌判定" prog. in Med. 15. 1862-1868 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ito G: "Faefars affecfing quality of ulcr hasling af ter Lomsoproyle trcatment" Jcein Gartroeuferal. 20(Suppl2). 62-66 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Furuta T: "Effects of Lansoprazole with or without amoxicillin on ulor healing:relation to eredication" Jcein Gartroeuferal. 20(Suppl2). 107-111 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Futami: "Quantitation of Helicobacter pylori using competitive polymerase chain reaction" 10th congress of Gastroenterology Abstracts II. 149- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 二見 肇: "Competitive PCR法を用いた消化性潰瘍患者における粘膜組織内Helicobacter pyloviの定量" 日本消化器内視鏡学会雑誌. 37. 114- (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 古田隆久: "合成DNAを用いたcompetitive PCR法による胃粘膜中helicobacter pyloviの定量" 日本消化器病学会雑誌. 91. 659- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Furuta: "Eftect of Laniscprizale with or without Hijh-Pole Aivoxicillin on Helscobcaten pylori in peptic ulcer patients" Suprelsion of Acid Secvetion by Lansoprazale and Ies Effect or lt pylor. 2. 73-75 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 古田隆久: "消化性潰瘍治療におけるPPI・抗生剤併用の意義" 日本消化器病学会雑誌. 91. 1703- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Furuta: "Quantificontion of Helicobacar pylni in gastsic inncus 7 conpetitive PCR wig synthetic DNA fragments" 10th Wovcl Cogrecs of Grastroencerology. 148- (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi