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Hormonal Regulation of Water and Electrolyte Transport in the Colon

Research Project

Project/Area Number 06670536
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

HANAI Hiroyuki  Hamamatsu University School of Medicine. First Dept. of Medicine. Assistant Professor., 医学部附属病院, 講師 (80208553)

Co-Investigator(Kenkyū-buntansha) KANEKO Eizo  Hamamatsu University School of Medicine. First Dept. of Medicine. Professor., 医学部, 教授 (10010414)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsVasopressin / Colon / V1 receptor / Cytosolic calcium / Sodium absorption / Potassium secretion / Chloride secretion / 小腸 / イオン輸送 / バソプレッシン / 水輸送 / 細胞内情報伝達 / 細胞内 pH
Research Abstract

Arginine vasopressin (AVP) plays an important role in fluid and electrolyte homeostasis, but its effect on intestinal transport is not fully elucidated. We examined the effect of AVP on colonic mucosal transport using Ussing chambers and cytosolic calcium concentration ([Ca^<2+>]_i) using fura-2 in guinea pig distal colonic epithelium. Serosally added 0.1 nM-1 mM-AVP caused concentration dependent changes in short circuit current (Isc) and tissue conductance (Gt). They include a bumetanide (0.1 mM,serosal)-sensitive increase in Isc which shows electrogenic Cl secretion, and a bumetanide (0.1 mM,serosal)-sensitive decrease in Isc which means electrogenic K^+ secretion. Both changes in Isc were associated with increases in Gt. On the other hand, AVP caused an amiloride (0.1 mM,mucosal)-sensitive decrease in Isc and Gt which coincided inhibition of electrogenic Na^+ absorption. AVP also caused biphasic increases in [Ca^<2+>]_i of crypt cells in a concentration dependent manner. [Ca^<2+>]_i in surface epithelial cells also responded to AVP.All these responses to AVP were abolished by a vasopressin V_1-antagonist. These studies indicate that AVP mobilizes [Ca^<2+>]_i via V_1-receptor, stimulates both bumetanide-sensitive K^+ and Cl^- secretion, and inhibits an amiloride-sensitive Na^+ absorption in guinea pig distal colon.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 佐藤嘉彦、花井洋行、金子榮藏他: "モルモット大腸粘膜イオン輸送に対するバソプレシンの作用" 消化と吸収. 18. 23-25 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takayanagi S.,Hanai H.,Kaneko E.et.al.: "Serotonin Uptake and Its Modulation in Rat Jejunal Enterocyte Preparation" Journal of Pharmacology & Experimetal Therapeutics. 272. 1151-1159 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 花井洋行、金子榮藏: "コレラ" 日本臨床領域別症候群シリーズ 消化管症候群(下). 6. 206-208 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 伊熊睦博、花井洋行、金子榮藏他: "加齢ラットにおける小腸吸収上皮細胞内pHの調節能" 消化と吸収. 17. 102-105 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshihiko Sato, Hiroyuki Hanai, Eizo Kaneko, Hisayoshi Hayashi, Yuichi Suzuki: "Effect of vasopressin on the electrolyte transport of guinea pig colonic epithelium" Digestion & Absorption. 18. 23-25 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shigekazu Takayanagi, Hiroyuki Hanai, Eizo Kaneko: "Serotonin Uptake and Its Modulation in Rat Jejunal Enterocyte Preparation" Journal of Pharmacology & Experimental Therapeutics.272. 1151-1159 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroyuki Hanai, Eizo Kaneko: "Cholera" Nippon Rinsho. Syndromes in Gastrointestinal Tract.6. 206-208 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mutsuhiro Ikuma, Hiroyuki Hanai, Eizo Kaneko, Hisayoshi Hayashi, Takeshi Hoshi: "Effects of Aging on the regulation of intracellular PH of rat small intestine" Digestion & Absorption. 17. 102-105 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 佐藤嘉彦、花井洋行、金子榮藏他: "モルモット大腸粘膜イオン輸送に対するバソプレシンの作用" 消化と吸収. 18. 23-25 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takayanagi S.,Hanai H.,Kaneko E.et.al.: "Serotonin Uptake and Its Modulation in Rat Jejunal Enterocyte Preparation" Journal of Pharmacology & Experimetal Therapeutics. 272. 1151-1159 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 花井洋行、金子榮藏: "コレラ" 日本臨床領域別症候群シリーズ 消化管症候群(下). 6. 206-208 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 伊熊睦博、花井洋行、金子榮藏他: "加齢ラットにおける小腸吸収上皮細胞内pHの調節能" 消化と吸収. 17. 102-105 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takayanagi Shigekazu: "Serotonin Uptake and Its Modulation in Rat Jejunal Enterocyte Preparation" J.Pharmacol.Exp.Ther.272(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 伊熊睦博: "加齢ラットにおける小腸吸収上皮細胞内pHの調節能" 消化と吸収. 17. 102-105 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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