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Analysis of humoral immune response to hypervariable region of hepatitis C virus

Research Project

Project/Area Number 06670537
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionNagoya University

Principal Investigator

YOSHIOKA Kentaro  Nagoya University School of Medicine, Instructor, 医学部, 助手 (60201852)

Co-Investigator(Kenkyū-buntansha) IWATA Kazuo  Nagoya University School of Medicine, Senior resident, 医学部, 医員
OKUMURA Akihiko  Nagoya University School of Medicine, Senior resident, 医学部, 医員
KAKUMU Shinichi  Nagoya University School of Medicine, Associate professor, 医学部, 講師 (10115545)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsglutathione S-transferase / hepatitis C virus / hypervariable region / anti-HVR antibody / 融合蛋白 / グルタチオン-S-トランスフェラーゼ / 慢性肝炎 / 抗体
Research Abstract

HVRs of 26 clones of six patients with chronic hepatitis C were expressed as proteins fused with glutathione S-transferase (GST), and sera of the patients were serially tested for antibody to these proteins. The extent of antibody response to HVR differed considerably among the patients. Three patients showed no or only scanty antibody response, and the others showed frequent or persistent antibody response. An augmentation of antibody response always followed the exacerbation of serum alanine aminotransferase levels. These results indicates that deficiency of antibody response to HVR may be one of the causes of persistency in HCV infection.
Subsequently, we investigated the antibody response to HVR in 21 patients with chronic hepatitis C.8-26 clones per serum were expressed, and the reactivity with the sera was assessed by Western blot. The incidence of HVR fusion proteins reactive with the corresponding serum was significantly higher in genotype 2a than in genotype 1b. This difference may be related to the distinctions in the clinical aspects of these two HCV genotypes.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Yoshioka K,Higashi Y,Tanaka K et al.: "Deficiency of antibody response to hypervariable region of hepatitis C virus in patients with chronic hepatitis C." J Hepatol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 吉岡健太郎、相山敏之、高柳正弘、奥村明彦、岩田和郎、各努伸一: "HCVエンベロープの高変異領域蛋白に対する血中抗体-GST融合蛋白を用いての検討。小俣政男監修。細胞内遺伝子導入と消化器疾患。" 国際医書出版, 7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshioka K,Higashi Y,Tanaka k et al.: "Deficiency of antibody responae to hypervariable region of hepatitis C virus in patients with chronic hepatitis C." J Hepatol. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] 吉岡健太郎、相山敏之、高柳正弘、奥村明彦、岩田和郎、各務伸一.: "HCVエンベロープの高変異領域蛋白に対する血中抗体-GST融合蛋白を用いての検討.小俣政男監修.細胞内遺伝子導入と消化器疾患." 国際医書出版,

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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