| Project/Area Number |
06670537
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Nagoya University |
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Principal Investigator |
YOSHIOKA Kentaro Nagoya University School of Medicine, Instructor, 医学部, 助手 (60201852)
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| Co-Investigator(Kenkyū-buntansha) |
IWATA Kazuo Nagoya University School of Medicine, Senior resident, 医学部, 医員
OKUMURA Akihiko Nagoya University School of Medicine, Senior resident, 医学部, 医員
KAKUMU Shinichi Nagoya University School of Medicine, Associate professor, 医学部, 講師 (10115545)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | glutathione S-transferase / hepatitis C virus / hypervariable region / anti-HVR antibody / 融合蛋白 / グルタチオン-S-トランスフェラーゼ / 慢性肝炎 / 抗体 |
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| Research Abstract |
HVRs of 26 clones of six patients with chronic hepatitis C were expressed as proteins fused with glutathione S-transferase (GST), and sera of the patients were serially tested for antibody to these proteins. The extent of antibody response to HVR differed considerably among the patients. Three patients showed no or only scanty antibody response, and the others showed frequent or persistent antibody response. An augmentation of antibody response always followed the exacerbation of serum alanine aminotransferase levels. These results indicates that deficiency of antibody response to HVR may be one of the causes of persistency in HCV infection.
Subsequently, we investigated the antibody response to HVR in 21 patients with chronic hepatitis C.8-26 clones per serum were expressed, and the reactivity with the sera was assessed by Western blot. The incidence of HVR fusion proteins reactive with the corresponding serum was significantly higher in genotype 2a than in genotype 1b. This difference may be related to the distinctions in the clinical aspects of these two HCV genotypes.
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