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Study on virus particle (s) in the blood of patients with hepatitis C : an evaluation of its pleomorphism

Research Project

Project/Area Number 06670539
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionMie University

Principal Investigator

WATANABE Shozo  Mie University, Health Administration Center, Professor, 保健管理センター, 教授 (20134934)

Co-Investigator(Kenkyū-buntansha) KAITO Masahiko  Mie University, School of Medicine, Assistant, 医学部, 助手 (70214244)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Keywordshepatitis C / hepatitis C virus (HCV) / HCV particle / immunogold electron microscopy / non-C hepatitis / virus-like particle
Research Abstract

In this study, we searched for HCV particle in the blood of patients with hepatitis C and found virus-like particles with pleomorphism in size and shape by the electron microscopic observation. First, we attempted to visualize HCV particle by indirect immunogold electron microscopy, using specific antibodies to the HCVE1 protein, and succeeded in identifying HCV virion in HCV-RNA-rich samples. HCV virious, immunologically discriminated from pleomorphic virus-like particles, were 55-65 nm spherical particles with delicate 6 nm-long surface projections. Subsequently, we detected the coincidence of antigenic activities of HCVE2/NS1 and HCVE1 proteins on the surface of HCV particle by the immunogold electron microscopy using anti-HCVE2/NS1 and/or anti-HCVE1 anti-bodies. This result indicates that both HCVE1 and HCVE2/NS1 proteins are components of the envelope of HCV virion, as presumed on the basis of the construct of HCV genome.
Our research also showed that pleomorphic virus-like particles, distinct from HCV,existed in the blood from hepatitis C patients and non-B,non-C hepatitis patients with high incidence. Prevalence of circulating pleomorphic virus-like particles was significantly correlated with the elevation in ALT levels. Although GBV-C-RNA was not detected in any samples containing these virus-like particles and genomic characterization has not been further achieved, it should be done to clarify the nature of these pleomorphic virus-like particles.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Masahiko Kaito,Shozo Watanabe,et al: "Hepatitis C virus particle detected by immunoelectronmicroscopic study." Journal of General Virology. 75(7). 1755-1760 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 垣内雅彦,渡邊省三,他: "C型肝炎ウイルス確認される." Medical Technology. 23(2). 95-97 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渡邊省三,垣内雅彦,他: "C型肝炎ウイルス粒子同定の試み." Pharma Medica. 13(3). 175-179 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渡邊省三,垣内雅彦,他: "C型肝炎ウイルスの免疫電子顕微鏡による探索とその形態学." 日本臨床. 53(8). 2069-2078 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渡邊省三,垣内雅彦,他: "免疫電顕法によるHCV粒子の超微形態と構造." 日本臨床. 53(増刊). 11-16 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渡邊省三: "C型肝炎ウイルスの免疫電子顕微鏡による同定の試み." 学術新報 Academia. No.169. 52-59 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渡邊省三,垣内雅彦,他: "最新アプローチC型肝炎-HCV解明からIFN療法の実際" 服部信,野本明男,小俣政男, 246 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masahiko Kaito, Shozo Watanabe, et al: "Hepatitis C virus particle detected by immunoelectron microscopic study." Journal of General Virology. 75(7). 1755-1760 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shozo Watanabe, Masahiko Kaito, et al: "Hepatitis C virus particle. In : Hepatitis C up-to-date (ed by Nobu Hattori, et al)" Medical View, Tokyo. (in Japanese). 16-21 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masahiko Kaito, Shozo Watanabe, et al: "Hepatitis C virus has been confirmed." Medical Technology. 23(2) (in Japanese). 95-97 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shozo Watanabe, Masahiko Kaito, et al: "An attempt to identify the hepatitis C virus particle." Pharma Medica. 13(3) (in Japanese). 175-179 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shozo Watanabe, Masahiko Kaito, et al: "Searching for hepatitis C virus by immunoelectron microscopy and its morphology." Japanese Journal of Clinical Medicine. 53(8) (in Japanese). 2069-2079 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shozo Watanabe, Masahiko Kaito, et al: "Ultrastructural feature of HCV particle as revealed by immunoelectron microscopy." Japanese Journal of Clinical Medicine. 53 (suppl) (in Japanese). 11-16 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shozo Watanabe: "An attempt to identify hepatitis C virus by immunoelectron microscopy." Academia. No.169 (in Japanese). 52-59 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渡邉 省三,垣内 雅彦,他: "C型肝炎ウイルスの免疫電子顕微鏡による探索とその形態学." 日本臨床. 53(8). 2069-2078 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 渡邉 省三,垣内 雅彦,他: "免疫電顕法によるHCV粒子の超微形態と構造." 日本臨床. 53(増刊). 11-16 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 渡辺省三: "C型肝炎ウイルスの免疫電子顕微鏡による同定の試み." 学術新報Academia. No.169. 52-59 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mashiko Kaito,Shozo Watanabe,et al。: "Hapatitis C virus particle detected by immunoelectron microscopic study" Journal of General Virology. 75(7). 1755-1760 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 渡邉 省三,垣内 雅彦,他: "C型肝炎ウイルス粒子同定の試み" Pharma Medica. 13(3). 175-179 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 渡邉 省三,垣内 雅彦,他: "最新アプローチC型肝炎-HCV解明からIFN療法の実際(第1章C型肝炎ウイルスの基礎-C型肝炎ウイルス粒子)" 服部信,野本明男,小俣政男監修,田中慧,小原道法編集(株式会社 メジカルビュー社), 246 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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