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Basic Study on DNA immunization against hepatitis C virus by using a transgenic mouse system

Research Project

Project/Area Number 06670580
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionJichi Medical School

Principal Investigator

MORIYAMA Takashi  Jichi Medical School, Liver Study Laboratory, Assistant Professor, 医学部, 講師 (10240706)

Co-Investigator(Kenkyū-buntansha) ANDO Kazuki  Jichi Medical School, Liver Study Laboratory, Assistant, 医学部, 助手 (20265276)
KANEKO Takashi  Jichi Medical School, Liver Study Laboratory, Assistant, 医学部, 助手 (10254913)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsHCV core protein / Cytotoxic T lymphocytes / DNA immunization
Research Abstract

Basic strategy for prevention of viral infection is, in general, to induce neutralizing antibody. This strategey, however, may not apply to hepatitis C,because there is no known neutralizing antibody that leads to the termination of infection. Cytotoxic T lymphocytes (CTL) also eradicates viral infection. Methods of inducing CTL is not as well established as those of inducing antibodies. A new method of inducing CTL is to use a naked DNA coding target antigens as an immunogen. The objective of this study is to test whether this technology is applicable to hepatitis C virus.
Mice were immunized with cDNA coding hepatitis C core region intramuscularly. Primed splenocytes showed a hepatitis C core protein specific proliferation. The splenocytes were also cultured with a cell line that expressed hepatitis C core protein. Cultured splenocytes showed specific CTL responses to hepatitis C core.
These results suggest that the DNA immunization method is applicable to hepatitis C virus antigens. For further analysis, transgenic mouse systems that express hepatitis C virus antigens at a higher level are under construction.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Ando,Kazuki: "Class I restricted cytotocic T lymphocytes aredirectly cytopathic" Journal of Immunology. 152. 3245-3253 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kita,Hiroto: "A helper T-cell antigen enhances generation of hepatitis Cvirus-specific cytotoxicx T lymphocytes in vitro." Journal of Medical Virology. 45. 386-391 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harase,Ichiro: "Immune response to hepatitis C virus core protein in mice." Immunology and Cell Biology. 73. 346-352 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kita,Hiroto: "HLA B44-restricted cytotoxic T lymphocyte responses to the pcptides of HCV nucleoprotein residues 81-100 in patients with chronic hepatitis C." Journal of Gastroenterology. 30. 809-812 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kita,Hiroto: "Aminimal and optimal cytotoxic T cell epitope within hepatitis Cvirus nucleoprotein." Jorunal of General Virology. 76. 3189-3193 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneko,Takashi: "Three new cytotoxic T cell epitopes identified within the hepatitis C virus nucleoprotein." Journal of General Virology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kita,Hiroto: "Springer-Verlag.In Viral Hepatitis and Liver Disease" Recognition of hepatitis C virus nucleocapsid protein-derived peptides by cytotoxic T lymphocytes., 4 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harase, Ichiro: "Immune response to hepatitis C virus core protein in mice" Immunology and Cell Biology. Vol.73. 346-352 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ando, Kazuki: "Class I restricted cytotocic T lymphocytes are directly cytopathic for their target cells in vivo." Journal of Immunology. 152. 3245-3253 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kita, Hiroto: "A helper T-cell antigen enhances generation of hepatitis C virus-specific cytotoxic T lymphocytes in vitro" Journal of Medical Virology. 45. 386-391 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Harase, Ichiro: "Immune response to hepatitis C virus core protein in mice" Immunlogy and Cell Biology. 73. 346-352 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kita, Hiroto: "HLA B44-restricted cytotoxic T lymphocyte responses to the peptides of HCV nucleoprotein residues 81-100 in patients with chronic hepatitis C" Journal of Gastroenterology. 30. 809-812 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kita, Hiroto: "A minimal and optimal cytotoxic T cell epitope within hepatitis C virus nucleoprotein" Jpurnal of General Virology. 76. 3189-3193 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaneko, Takeshi: "Three new cytotoxic T cell epitopes identified within the hepatitis C virus nucleoprotein." Journal of General Virology. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kita, Hiroto: "Springer-Verlag. In Viral Hepatitis and Liver Disease" Recognition of hepatities C virus nucleocapsid protein-derived peptides by cytotoxic T lymphocytes., 4 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroto,Kita: "A helper T-cell antigen enhances generation of hepatitis C virus-specific cytotoxic T lymphocytes in vitro" Journal of Medical Virology. (in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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