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Multifactorial analysis of chronicity and pathogenesis in patients with sarcoidosis

Research Project

Project/Area Number 06670610
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAGAI Sonoko  Chest Disease Research Institute, Kyoto University Associate Professor, 胸部疾患研究所, 助教授 (30217955)

Co-Investigator(Kenkyū-buntansha) SATAKE Norio  Chest Disease Research Institute, Kyoto University Assistant Professor, 胸部疾患研究所, 助手 (50252515)
KITAICHI Masanori  Chest Disease Research Institute, Kyoto University Associate Professor, 胸部疾患研究所, 助教授 (00161464)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsSarcoidosis / T cell antigenic receptor (TCR) / HLA-typing / Japanese / Swedish / Flow cytometry / Monoclonal Antibody / TCR V gene / スウエーデン人 / TCRVβ22、CD8陽性T細胞 / HLAB61(40)
Research Abstract

The clinical features of sarcoidosis are heterogenous in different ethnic groups, suggesting that different genetic or environmental backgrounds influences the disease. For comparison between Japanese and Swedish in whom a correlation between lung accumulated CD4 Tcells expressing the T cell receptor (TCR) V_<alpha> 2.3 gene segment and a particular HLA type (DR3 (17), DQ2) was shown, we examined TCR V gene usage and gammadelta TCR expression in CD4 and CD8 T cells in BAL fluid and peripheral blood obtained from Japanese sarcoidosis patients and healthy controls. We used a panel of 13 monoclonal antibodies (Mab) specific for different TCR V genes, which altogether stained approximately 50% of the T cells, and triple staining techniques with flow cytometry. The patients and controls were also HLA-typed. As results, a high degree of expression of gammadelta TCR in blood T cells of close to half of the patients. Expansion of T cell subsets was readily detected in the CD8 T cell population, while a more homogenous staining pattern was found in the CD4 T cell population. On the other hand, the pattern of reactivities found in CD4 and CD8 subsets of blood lymphocytes of Japanese healthy subjects was in general found to be match that in healthy Caucasian subjects. It remains to be discussed whether influence of ethnic factors on TCR gene expression of lung T cells is greater than that of environmental ones, in the case of diseases processes such as sarcoidosis.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Shigematsu M et al: "T cell receptor(TCR)V gene segment use in HLA-typed japanese healty suljects" Clin Exp Immunol. 103. 149-154 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Grunewald J et al: "T cell receptor V gene expression in HLA-typed Japanese patients with pulomonary sarcoidosis" Am J Respir Crit Med. 151. 151-156 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki Y et al: "Analysis of fused-membrane structures in bronchoalveolar lavage fluid from patients with alveolar proteinosis" Am J Respir Cell Mol Biol. 12. 238-249 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Guzman J et al: "Immunocytochemical characterization of isolated human type II pneumocytes" Acta Cytologica. 38. 539-542 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Guzman J et al: "ICAM-1 and integrin expression on isolated human alveolar type II pneumocytes" Eur Respir J. 7. 736-739 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi T et al: "Soluble interleukin-2 receptor in blood from patients with sarocidosis and idiopathic pulmonary fibrosis" Sarcoidosis. 11. 102-109 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai S.: "Pulmonary Sarcoidosis; Population differences and pathophysiology." Southern Med J.88-10. 1001-1010 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai S.: "Pulmonary Sarcoidosis; Pathogenesis and population differences" Internal Medicine. 34-9. 833-838 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishimura K,: "CT and pathological correlation of pulmonary sarcoidosis." Seminars in Ultrasound, CT and MRI. 16-5. 361-370 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai S et al: "Conn′s Current Therapy 1995" W. B. Saunders, 1204 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shigematsu M.: "T cell receptor (TCR) V gene segment use in HLA-typed Japanese healthy subjects." Clin Exp Immunol. 103-2. 149-154 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Grunewald J.: "T cell receptor V gene expression in HLA-typed Japanese patients with pulmonary sarcoidosis." Am J Respir Crit Care Med. 151-1. 151-156 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki Y: "Analysis of fused-membrane structures in bronchoalveolar lavage fluid from patients with alveolar proteinosis." Am J Respir Crit Care Med. 12-2. 238-249 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Guzman J: "Immunocytochemical characterization of isolated human type II pneumocytes" Acta Cytologica. 38-4. 539-542 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Guzman J: "ICAM-1 and integrin expression on isolated human alveolar type II pueumocytes" Eur Respin. J 7-6. 736-739 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsumi T: "Soluble interleukin-2 receptor in blood from patients with sarcoidosis and idiopathic pulmonary fibrosis." Sarcoidosis. 11-2. 102-109 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai S: "Pulmonary Sarcoidosis ; Population differences and pathophysiology." Southern Med J.88-10. 1001-1010 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai S.: "Pulmonary Sarcoidosis ; Pathogenesis and population differences" Internal Medicine. 34-9. 833-838 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishimura K: "CT and pathological correlation of pulmonary sarcoidosis." Seminars in Ultrasound, CT and MRI. 16-5. 361-370 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai S: Sarcoidosis. W.B.Saunders, Conn's Current Therapy, 1204 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shigematsu M et al: "T cell receptor(TCR)V gene segment use in HLA-typed Japanese healthy subjects" Clin Exp Immunol. 103. 149-154 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Grunewald J et al: "T cell receptor V gene expression in HLA-typed Japanese patients with pulmonary sarcoidosis" Am J Respir Crit Care Med. 151. 151-156 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki Y et al: "Analysis of fused-membrane structures in bronchoalveolar lavage fluid from patients with alveolar proteinosis" Am J Respir Cell Mol Biol. 12. 238-249 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Guzman J et al: "Immunocytochemical characterization of isolated human type II pneumocytes" Acta Cytologica. 38. 539-542 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Guzman J et al: "ICAM-1 and integrin expression on isolated human alveolar type II pneumocytes" Eur Respir J. 7. 736-739 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsutsumi T et al: "Soluble interleukin-2 receptor in blood from patients with sarcoidosis and idiopathic pulmonary fibrosis" Sarcoidosis. 11. 102-109 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagai S, et al: "Conn's Current Therapy 1995" W. B. Saunders, 1204 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Grunewald J et al: "T cell receptor V gene expression in HLA-typed Japanese patients with pulmonary sarcoidosis" Am J Respir Crit Care Med. 151. 151-156 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki Y et al: "Analysis of fused-membrane structures in bronchoalveolar lavage fluid from patients with alveolar proteinosis" Am J Respir Cell Mol Biol. 12(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Guzman J et al: "Immunocytochemical characterization of isolated human type II pneumocytes" Acta Cytologica. 38. 539-542 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Guzman J et al: "ICAM-1 and integrin expression on isolated human alveolar type II pneumocytes" Eun Respir J. 7. 736-739 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsumi T et al: "Soluble interleukin-2 receptor in blood from patients with sarcoidosis and idiopathic pulmonary fibrosis" Sarcoidosis. 11. 102-109 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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