• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

STUDIES ON ANTIGEN-SPECIFIC T-LYMPHOCYTE IN BRONCHIAL ASTHMA

Research Project

Project/Area Number 06670613
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

TADA Shinya  OKAYAMA UNIVERSITY,DEPARTMENT OF MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (20135982)

Co-Investigator(Kenkyū-buntansha) KANEHIRO Arihiko  OKAYAMA UNIVERSITY,UNIVERSITY HOSPITAL,INSTRUCTOR, 医学部・附属病院, 助手 (20243503)
KIMURA Goro  OKAYAMA UNIVERSITY,UNIVERSITY HOSPITAL,INSTRUCTOR, 医学部・附属病院, 助手 (90252957)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBRONCHIAL ASTHMA / ANTIGEN-SPECIFIC T-CELL / ANIMAL MODEL OF BRONCHIAL ASTHMA / ASCARIS ANTIGEN / BRONCHIAL HYPERRESPONSIVENESS / LYMPHOCYTE BLASTOGENESIS / リンパ球 / 遅発型気道反応
Research Abstract

To investigate the role of antigen-specific T-lymphocyte in bronchial asthma, guinea pigs were actively sensitized by intraperitoneal injection of ascaris antigen with hydrated alumina and provoked bronchial reactions by inhalation of aerosolized ascaris antigen. Proliferation of peripheral blood lymphocytes (PBL) with ascaris antigen was significantly higher in sensitized guinea pigs compared with control. But there was no correlation between proliferation of lymphocytes and bronchial hyperresponsiveness or incidence of immediate asthmatic responses (IAR).
In human, antigen specific T-cell clones were derived from allergic asthmatics. IFN-_Y or IL-4 was secreted from these clones. Non-specific T-cell clone derived from asthmatics who had no specific IgE showed various secretion patterns of IFN-_Y, IL-4 and IL-5. In ascaris-sensitized guinea pig, proliferation of lymphocyte was observed when PBL were cultured with ascaris antigen. These antigen-specific cell lines proliferated with addition of rhIL-2.
These findings suggested that ascaris-specific T-cell clones can be established. And influence of antigen specific T-cell clone on IAR or late asthmatic response can be examined by returning autologous expanded ascaris-specific T-cell clone to original guinea pig.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 辻 晃仁: "連日抗原吸入による実験的慢性喘息におけるEicosanoidsの役割に関する研究" 岡山医学会雑誌. 106. 825-935 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宮原信明: "気管支喘息における抗原特異的T-cell cloneの樹立とサイトカイン産生能に関する研究" 岡山医学会雑誌. 106. 907-915 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 金廣有彦: "難治病態とアラキドン酸カウケード-慢性気道炎症との関連" 難治病態とアラキドン酸カスケード. 14-22 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木村五郎: "BAL所見からみた喘息発作死のリスクファクターについて" Asthma-an infla mmatory disease. 1. 64-66 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 多田慎也: "リンパ球Subsetsの動態" アレルギーの領域. 2. 45-47 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akihito Tuji: "The role of eicoxanoids in airway hyperreponsiveness of chronic asthma using an animal model." Okayama Igakukai Zasshi. 106. 825-835 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nobuaki Miyahara: "Establishment of antigen-specific T-cell clones from asthmatic patients including evaluation of cytokine production." Okayama Igakukai Zasshi. 106. 907-915 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Arihiko Kanehiro: "Intractable state and arachidonic acid-relation with chronic inflammation." Nantibyoutai to arakidonsan Kasukedo. 14-22 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Goro Kimura: "BALF findings as risk factor of asthma death." Astham-an inflammatory disease. 1. 64-66 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shinya Tada: "Subsets of lymphocytes." Arerugi no ryouiki. 2. 455-47 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 木村五郎: "難治性喘息Tリンパ球のクローン化とサイトカイン産性能" アレルギー. 44. 283 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 木村五郎: "喘息の病態と喘息死との関係" アレルギー. 44. 863 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 金廣有彦: "難治病態とアラキドン酸カスケード-慢性気道炎症との関連" 難治病態とアラキドン酸カスケード. 14-22 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 木村五郎: "BAL所見からみた喘息発作死のリスクファクターについて" Asthma-an inflammatory disease. 1. 64-66 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 多田慎也: "リンパ球subsetsの動態" アレルギーの領域. 2. 45-47 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 辻.晃仁: "連日抗原吸入による実験的慢性喘息におけるEicosanoldsの役割に関する研究" 岡山医学会雑誌. 106. 825-830 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 宮原信明: "気管支喘息における抗原特異的T-cell cloneの樹立とサイトカイン産生能に関する研究" 岡山医学会雑誌. 106. 907-915 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi