Screening of new KL-6-associated mucins useful in clinical diagnosis of interstitial pneumonitis
| Project/Area Number |
06670618
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | EHIME UNIVERSITY |
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Principal Investigator |
KOHNO Nobuoki School of Medicine, Lecturer, 医学部・附属病院, 講師 (80215194)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Akihito School of Medicine, Assistant professor, 医学部, 助手 (30191513)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | KL-6 / KL-6 sub-molecules / interstitial pneumoitis / EK-7 antibody / PEP-76 / モノクローナル抗体 / 血清マーカー / ムチン / MUC1 / クラスター9 |
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| Research Abstract |
Screening of murine monoclonal antibodies which react with novel antigenic deternlinants on KL-6 molecules was performed last year.
EK-7 monoclonal antibody was finally selected for the purpose this year.
EK-7 antibody was IgM and reacted with an antigenic determinant different from the determinant recognized with KL-6 antibody. The antigenic determinant was peptide portion of KL-6 molecules.
The PEP-76 molecule in serum which was detected by a sandwich type of enzyme immunoasssay using EK-7 antibody as a catcher and KL-6 antibody as a tracer was designated as PEP-76. The method using 96-well EIA plates was similar to that for the measurement of KL-6.
Study population was 51 healthy individuals, 13 patients with idiopathic interstitial pneumonia and 12 patients with sarcoidosis.
PEP-76 level was 0.9 (0.9-3.9) U/ml [median (range)] in healthy individuals, 54 (0.9-580) U/ml in idiopathic interstitial pneumonia, and 1 (0.9-13) U/ml in sarcoidosis. Both the levels in idiopathic interstitial pneumonia and sarcoidosis were statistically higher than that in healthy individuals. when the cut-off level of PEP-76 was determined at 2.7 U/ml from the distribution of the levels of healthy individuals, the positive rate was 92% in idiopathic interstitial pneumonia and 33% in sarcoidosis. The sensitivity of PEP-76 was higher than that of KL-6 in same sera from patients with interstitial pneumonitis.
These observations indicate that PEP-76 is a novel serum marker for the diagnosis of interstitial pneumonitis.
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Report
(3 results)
Research Products
(19 results)
