| Project/Area Number |
06670655
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Kagawa medical school |
|---|
Principal Investigator |
TAKEUCHI Hiroaki Kagawa medical school, Undergraduate school of medicine, Assistant professor, 医学部, 助教授 (40112049)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
DEGUCHI Kazushi Kagawa medical school, School hospital, Assistant, 医学部附属病院, 助手 (80263896)
TOUGE Tetsuo Kagawa medical school, School hospital, Assistant, 医学部附属病院, 助手 (80197839)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | dopaminergic neurons / substantia nigra pars compacta / levodopa / terminal excitability / 黒質線条件ドーパミンニューロン / L-Dopa |
|---|
| Research Abstract |
In large doses, levodopa decreases the firing rate of rat dopaminergic neurons in the substantia nigrapars compacta (SNC DA). This phenomenon may be due to a negative feedback mechanism through autoreceptors within the substantia nigra. Whether chronic levodopa administration influences on SNC DA remain unclear. We evaluated SNC DA function using the firing rate and dopaminergic terminal excitability of dopamine neurons in male Sprague-Dawley rats. Two groups of animals ; the first group (n=19 rats) received daily intraperitoneal injection of levodopa (100mg/kg) and carbidopa (25mg/kg) for 60 days, while the second group (n=27) was the control. Extracellular single unit recordings of SNC DA were obtained with micropipettes. Dopaminergic terminal excitability was determined as the threshold of antidromic responses evoked by neostriatal electrostimulation. There were no significant differences in the firing rate between two groups, but the levodopa group showed a decrease in the terminal excitability compared with that in the control group. The decrease of terminal excitability may be related to impare striatal dopamine release. This finding appears to be related the clinical observation that L-dopa therapeutic response diminishes following chronic treatment.
|
