Molecular Cloning and Chromosomal Localization of Human Hippocalcin Gene.
| Project/Area Number |
06670675
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Toho University |
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Principal Investigator |
TAKAMATSU Ken Toho University, School of Medicine, Professor, 医学部, 教授 (90154898)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Hippocalcin / Calcium-binding protein / Brain / Hippocampus / Gene / Chromosomal localization / cDNA |
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| Research Abstract |
Hippocalcin is a 23 kDa calcium-binding protein with a three EF-hand structure, primarily idenditified in the rat Hippocampus. Hippocalcin has a sequence homology with recoverin, suggesting that hippocalcin may regulate phosphorylation of receptor molecules in the hippocampus. To investigate a possible relationship of hippocalcin to human neurological disorders, we have analyzed the human hippocalcin gene. A human Hippocampus cDNA library was screened with a rat hippocalcin cDNA probe. Eight independent cDNA clones were obtained and classified into five species (hHLP1-5) based on the restriction enzyme maps. The clone hHLP1 encode the entire open reading frame of human hippocalin. The predicted amino acid sequence was 100% homologous to that of rat hippocalcin. After screening a human genomic DNA cosmid libray, we obtained 3 independent clones. All these 3 clones exhibited similar restriction fragment patterns. The human hippocalcin gene spans approximately 15-kb and contains 3 exons. The exon sequence of the human hippocalcin gene perfectly matched its cDNA sequence, and all exon-intron junctions conformed to the GT/AG rule. The intron positions are not consistently placed with respect to the coding regions of the tandemly repeated EF-hand domains. Southern blot analysis of the human genomic DNAs and cosmid clones with a human hippocalcin cDNA probe indicated that the human hippocalcin gene is present as a single copy gene in the haploid genome. The transcription initiation site was determined by primer extension assay with poly (A) + RNA from human brain and was assigned to the adenine residue at the position -181. In 2-kb sequence upstream of the transcription initiation site, no common motifs for mammalian promoters such as TATA-, GC- and CAAT-boxes appear to be present. By direct R-banding fluorescence in situ hybridization, the human hippocalcin gene was assigned to bands 1p34.2-35.
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Report
(3 results)
Research Products
(26 results)
