• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Human angiotensin II type-1 receptor gene and regulation.

Research Project

Project/Area Number 06670742
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionDokkyo University

Principal Investigator

FURUTA Hiroaki  Dokkyo University School of Medicine Lecturer, 医学部, 講師 (70049253)

Co-Investigator(Kenkyū-buntansha) MATSUZAKI Shigeru  Dokkyo University School of Medicine Professor, 医学部, 教授 (60008604)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsAngiotensin / Hormone receptor / Gene analysis / Expression / 遺伝子解析 / ホルモン受容体 / アンジテンシン
Research Abstract

Our studies were focused on the determination of the genomic organization of human angiotensin II type-1 receptor (AT_1) gene. The presence of five exons for human AT_1 gene and the first four exons encoding 5'untranslated DNA sequences were found from the screenings of the cDNAs. Each positive clone had different exons that were used to construct the map of human AT_1 gene including exon/intron junction sites. Typical intron donor and acceptor sites were found at all the borders of the exons. Sequencing results suggest that the 5'-untranslated region of human AT_1 gene consists of four exons. As seen in all the alternatively spliced human AT_1 transcripts, those transcripts encode the same and the single receptor suggesting the presence of a single functional promoter which contains the first exon. Analysis of the genomic DNA sequence upstream of the first exon showed several sequence motifs revealed in the promoters of the various genes ; Sp1 with two different types of GC boxes, two CAAT boxes, AP-2.Pit-1 (a pituitary transcriptional activator), and albumin-negative factor (ANF). Several cis-acting regulatory elements were also characterized. The results of functional analysis of the 5'-flanking region suggest positive and negative regulations of the transcription.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] H.Furuta: "Molecular Cloning and Sequencing of the Gene Encoding Human Angiotensin II Type-1 Receptor" Biochem.Biophys.Res.Commun.183・1. 8-13 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Inagami: "Cloning,Expression and Regulation of Angiotensin II Receptors" J.Hypertens.10・8. 713-716 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] R.Takayanagi: "Molecular Cloning,Sequencing Analysis and Expression of a cDNA Encoding Human Type-1 Angiotensin II Receptor" Biochem.Biophys.Res.Commun.183・2. 910-916 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Yoshida: "Analysis of the Evolution of Angiotensin II type-1 Receptor Gene in Mammals(Mouse,Rat,Bovine and Human)" Biochem.Biophys.Res.Commun.186・2. 1042-1049 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D-F.Guo: "The Genomic Organization of Human Angiotensin II Type 1 Receptor" Biochem.Biophys.Res.Commun.200・1. 313-319 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Inagami, N.Iwai, K.Sasaki, Y.Yamano, S.Bardhan, S.Chaki, DF.Guo, H.Furuta, K.Ohyama, Y.Kambayashi, K.Takahashi, T.Ichiki: "Cloning, expression and regulation of angiotensin II receptors" Eur.Heart J.15 (Suppl.D). 104-107 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] DF.Guo, H.Furuta, M.Mizukoshi, T.Inagami: "The genomic organization of Human angiotensin II type 1 receptor" Biochem.Biophys.Res.Commun.200 (1). 313-319 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Furuta: "Molecular Cloning and Sequencing of the Gene Encoding Human Angiotensin II Type-1 Receptor" Biochem.Biophys.Res.Commun.183・1. 8-13 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Inagami: "Cloning,Expression and Regulation of Angiotensin II Receptors" J.Hypertens.10・8. 713-716 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] R.Takayanagi: "Molecular Cloning,Sequencing Analysis and Expression of a cDNA Encoding Human Type-1 Angiotensin II Receptor" Biochem.Biophys.Res.Commun.183・2. 910-916 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Yoshida: "Analysis of the Evdution of Angiotensin II type-1 Receptor Gene in Mammals (Mouse,Rat,Bovine and Human)" Biochem.Biophys.Res.Commun.186・2. 1042-1049 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] D-F.Guo: "The Genomic Organization of Human Angiotensin II Type 1 Receptor" Biochem.Biophys.Res.Commun.200・1. 313-319 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Furuta: "Molecular Cloning and Sequencing of the Gene Encoding Human Angiotensin II Type-1 Receptor" Biochem. Biophys. Res. Commun.183. 8-13 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Inagami: "Cloning, Expression and Regulation of Angiotensin II Receptors" J. Hypertens.10. 713-716 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] R. Takayanagi: "Molecular Cloning, Sequencing Analysis and Expression of a cDNA Encoding Human Type-1 Angiotensin II Receptor" Biochem Biophys. Res. Commun.183. 910-916 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] H. Yoshida: "Analysis of the Evolution of Angiotensin II Type-1 Receptor Gene in Mammals (Mouse, Rat, Bovine and Human)" Biochem Biophys. Res. Commun.186. 1042-1049 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] M. Ishida: "Establishment of Chinese Hamster Ovary Cell Lines Stably Expressing the Cloned Human Type-1 Angiotensin II Receptor and Characterization of the Exprossed Receptor" Biomed. Res.13. 349-356 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Hamakubo: "A Na Pump Inhibitor from Bovine Posterion Pituitary: Purification, Structure Determination and its Cardiovascular Effect in Rat" Biochem. Biophys. Res. Commun.189. 691-696 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Furuta: "Molecular Cloning and Sequencing of the Gene Encoding Human AngiotesinII type-1 Receptor" Biochem.Biophys.Res.Commun.183. 8-13 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Inagami: "Cloning,Expression and Regulation of AngiotensinII Receptors" J.Hypertens.10. 713-716 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] R.Takayanagi: "Molecular Cloning,Sequencing Analysis and Expression of a cDNA Encoding Human Type-1 AngiotensinII Receptor" Biochem.Biophys.Res.Commun.183. 910-916 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Yoshida: "Analysis of the Evolution of AngiotensinII Type-1 Receptor Gene in Mammals (Mouse,Rat,Bovine and Human)" Biochem.Biophys.Res.Commun.186. 1042-1049 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Ishido: "Establishment of Chinese Hamster Ovary Cell Lines Stably Expressing the Cloned Human Type-1 AngiotensinII Receptor and Characterization of the Expressed Receptor" Biomed.Res.13. 349-356 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Hamakubo: "A Na Pump Inhibitor from Bovine Posterior Pituitary:Purification,Structure Determination and its Cardiovascular Effect in Rat" Biochem.Biophys.Res.Commun.189. 691-696 (1192)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi