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A study on the mechanism of transformation of the hypertrophied myocardium in infants, children and adults

Research Project

Project/Area Number 06670746
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionTokyo Women's Medical College

Principal Investigator

NISHIKAWA Toshio  Tokyo Women's Medical College, Associate Professor, 医学部, 助教授 (50120019)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Kazuaki  Iwate Medical College, Assistant Professor, 医学部, 講師 (30119888)
ANDO Akiko  Tokyo Women's Medical College, Assistant, 医学部, 助手 (90232090)
MASUDA Masahiro  Tokyo Women's Medical College, Assistant Professor, 医学部, 講師 (60209434)
KASAJIMA Takeshi  Tokyo Women's Medical College, Professor, 医学部, 教授 (30045653)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHypertrophied myocardium / Transformation / Cytoskelton / Interstitial cell / Immunohistochemistry / Molecular biology
Research Abstract

This study was designed to investigate the the morphological change and the desmin distribution in the hypertrophied myocytes and interstitial cells in the diseased heart of human and experimental rats by the methods of histopathology, immunohistochemistry and molecular biology. Light-microscopical examination revealed myocyte hypertrophy, disarrangement of muscle bundle and interstitial fibrosis in dilated cardiomyopathy of infants and children. Electronmicroscopy revealed scarcity of myofibrils, mitochondriosis and proliferation of rough-surfaced endoplasmic reticulum and Golgi apparatus. The latter findings may suggest an increase in protein synthesis in the myocyte. Similar aspects except for scarcity of myofibrils were observed in the myocardium from patients with Tetralogy of Fallot. Desmin filaments were increased in number and characterized by a disordered arrangement in the hypertrophied myocytes in dilated cardiomyopathy. Desmin immunolabeling was transversely and longitudina … More ly distributed at the Z band level in the cytoplasm of these cells. Myocytes with disordered arrangement of desmin showed distinct expression of natriuretic peptides, while negative expression of them was revealed in the myocytes with normally arranged desmin. In situ hybridization showed increase in expression of desmin mRNA in the right ventricle of the pulmonary artery-binding rat heart, where mRNA of natriuretic peptides or alpha-skeletal muscle actin was also expressed. Interstitial fibroblastic cells proliferated in the myocardial tissue in dilated cardiomyopathy, dilated-phase hypertrophic cardiomyopathy, myocardial infarction and congenital heart disease with ventricular dilatation showed positive immunoreactivity for alpha-smooth muscle actin. They also revealed positive reactivity for embryonic isoform of non-muscle myosin heavy chain. These cells are suggested to be myofibroblasts by the ultrastructural aspects including filament insersion site, abundant actin filaments and discontinuous basement membranes. These results indicate that hypertrophied myocytes show various morphological changes with transformation and interstitial fibroblastic cells also transform in the myocardial tissue of the diseased heart in infants, children and adults. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Michiaki Hiroe: "Distribution of desmin filaments in the myocardium of the failing heart." Medical Electron Microscopy. 27. 246-248 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 西川俊郎: "拘束型病態を示す心疾患" 循環器科. 36. 306-312 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masato Tanaka: "Cellular localization and structural characterization of natriuretic peptide-expressing ventricular myocytes from patients with dilated cardiomyopathy." J Histochem Cytochem. 42. 1207-1214 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Nishikawa: "Light and electron microscopic localization of inducible nitric oxide synthase in the heart of rats with myocarditis." J Microscopy Society Amer. 1. 708-709 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masato Tanaka: "Differential localization of atrial natriuretic peptide and skeletal alpha-actin messenger RNAs in left ventricular myocytes of patients with dilated cardiomyopathy." J Am Coll Cardiol. 26. 85-92 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshinori Seko: "Restricted usage of T cell receptor Vα-Vβ genes in. infiltrating cells in the heart with acute myocarditis and dilated cardiomyopathy." J Clin Invest. 96. 1035-1041 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Nishikawa: "Cardiomyopathy Update 5 Prognosis and Treatment of Cardiomyopathies and Myocarditis." Morie Sekiguchi and Peter J.Richardson University of Tokyo Press, 412 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 西川俊郎: "心臓病の自然歴" 広沢弘七郎、医学書院, 722 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Nishikawa, et al.: "Dilated cardio myopathy in infants and children : Histopathologic aspects of endomyocardial biopsy." Cardiomyopathy Update. 5. 181-186 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Michiaki Hiroe, et al.: "Distribution of desmin filaments in the myocardium of the failing heart." Medical Electron Microscopy. 27 (3-4). 246-248 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Nishikawa, et al.: "Heart disease with restricted physiology" Junkankika. 36 (4). 306-312 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masato Tanaka, et al.: "Cellular localization and structural characterization of natriuretic peptide-expressing ventricular myocytes from patients with dilated cardiomyopathy." J Histochem Cytochem. 42 (9). 1207-1214 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshio Nishikawa, et al.: "Light and electron microscopic localization of inducible nitric oxide synthase in the heart of rats with myocarditis." J Microscopy Society Amer. 1. 708-709 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masato Tanaka, et al.: "Differential localization of atrial natriuretic peptide and skeletal alpha-actin messenger RNAs in left ventricular myocytes of patients with dilated cardiomyopathy." J Am Coll Cardiol. 26. 85-92 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshinori Seko, et al.: "Restricted usage of T cell recepter Valpha-Vbeta genes in infiltrating cells in the heart with acute myocarditis and dilatedcardiomyopathy." J Clin Invest. 96. 1035-1041 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masato Tanaka: "Differential localization of atrial natriuretic peptide and skeletal alpha-actin messenger RNAs in left ventricular myocytes of patients with dilated cardiomyopathy." J Am Coll Cardiol. 26. 85-92 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshinori Seko: "Restricted usage of T cell receptor Vα-Vβ genes in infiltrating cells in the heart with acute myocarditis and dilated cardiomyopathy." J Clin Invest. 96. 1035-1041 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshio Nishikawa: "Light and electron microscopic localization of inducible nitric oxide synthase in the heart of rats with myocarditis" J Microscopy Society Amer. 1. 708-709 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshio Nishikawa: "The effect of forskolin on the teratogenicity of methylxanthines in the chick embryo heart." Reprod Toxicol. 9. 165-168 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuyo Takeda: "Apoptosis and DNA fragmentation in the bulbus cordis of the developing rat heart." J Mol Cell Cardiol. 28. 209-215 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 西川俊郎: "心臓病の自然歴。" 広沢弘七郎 医学書院, 722 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshio Nishikawa: "Dilated cardiomyopathy in infants and children:Histopathologic aspects of endomyocardial biopsy." Gardiomyopathy Update. 5. 181-186 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toshio Nishikawa: "Myofibroblasts expressing smooth muscle actin in the human myocardium of various heart diseases." J.Histochem.Cytochem.42. 983 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 西川俊郎: "拘束型病態を示す心疾患。" 循環器科. 36. 306-312 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masato Tanaka: "Cellular localization and structural characterization of natriuretic peptide-expressing myocytes from dilated cardiomyopathy." J.Histochem.Cytochem.42. 1207-1214 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masato Tanaka: "Hypoxia induced apoptosis with enhanced expression of Fas antigen mRNA in cultured neonatal rat cardiomyocytes." Circulation Research. 75. 426-433 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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