| Project/Area Number |
06670773
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | TOKYO MEDICAL AND DENTAL UNIVERSITY |
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Principal Investigator |
TAKAGI Shuji TOKYO MEDICAL AND DENTAL UNIVERSITY,DEPARTMENT OF PEDIATRICS,ASSISTANT PROFESSOR, 医学部, 講師 (20201593)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | EGG ALLERGY / CYTOKINES / T CELL CLONE / インターロイキン4 / インターフェロン・ガンマ |
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| Research Abstract |
Peripheral blood lymphocytes (PBL) from child with egg hypersensitivity proliferated in response to egg white, ovalbumin as well as obomucoid, and produced significantly more IL-4 than PBL from control chidren. Stimulation with PHA induced more IL-4-producing cells than the control. PBL from patients with atopic dermatitis produced less IFN-gamma than PBL from patients with isolated IgE-mediated egg hypersensitivity. Ovalbumin-specific CD4^+ T cell clones established from patients with atopic dermatitis produced IL-4, but no, or a limited amount of IFN-gamma. A clone responded to obalbumin peptide 323-339. Peptide PH-1.0 (Leu-Tyr-Gln-Glu-Leu-Gln-Lys-Leu-Thr-Gln-Thr-Leu-Lys) suppressed the binding to the lymphoblastoid cell line of peptide 323-339 and inhibited antigen presentation in a dose dependent manner.
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