| Project/Area Number |
06670782
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
SHIMOZAWA Nobuyuki GIFU UNIVERSITY,SCHOOL OF MEDICINE,DEPARTMENT OF PEDIATRICS,ASSISTANT PROFESSOR, 医学部附属病院, 講師 (00240797)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | PEROXISOME / ZELLWEGER SYNDROME / MODEL MOUSE / CHINESE HAMSTER OVERY CELL / CHO細胞 / 神経細胞移動障害 |
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| Research Abstract |
(1) Mouse peroxisome assembly factor-1 (PAF-1) genomic DNA was isolated for the purpose of making a peroxisome deficient model mouse by gene targetting.
(2) Rat PAF-2 cDNA was isolated by functional complementation of peroxisome deficiency of a mutant chinese hamster overy (CHO) cell, using transient transfection assey. PAF-2 cDNA complemented the peroxisomes of the fibroblasts derived from patients with Zellweger syndrome. Furthermore, we cloned human PAF-2 cDNA,and demonstrated the mutations of PAF-2 in the Zellweger patients.
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