| Project/Area Number |
06670784
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
ITO Masahiro Mie University School of Midicin, Department of Pediatrics Associate Professor, 医学部, 助教授 (10126956)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Cytomegalovirus / Adhesion moleacles / Cytotoxicity / ICAM-1 CCO541 / CD29 / CD44 |
|---|
| Research Abstract |
We investigated the adherence of peripheral blood leukocytes to cytomegalovirus (CMV) -infected and uninfected fibroblasts. Adherence of fresh peripheral blood mononuclear cells (PBMC) to CMV-infected fibroblasts was significantly greater than their adherence to uninfected fibroblasts. In leukocyte populations, fresh monocytes and B cells adhered to CMV-infected fibroblasts, whereas fresh T cells and polymorphonuclear leukocytes (PMNL) did not adhere to either CMV-infected or uninfected fibroblasts. When T cells were activated with anti-CD3 antibody, activated T cells demonstrated the adherence and cytotoxicity to both CMV-infected and uninfected fibroblasts. Magnitude of cytotoxicity mediated by adherent activated T lymphocytes was significantly greater that that of unifected fibroblasts. Adherence of PBMC and cytotoxicity mediated by adherent activated Tcells were blocked by treatment of CMV-infected fibroblasts with anti-ICAM-1 antibody and by treatment of leukocytes with anti-LFA-1 antibody. These data suggest that an interaction of ICAM-1 and LFA-1 is responsible for the adherence oc leukocytes and for adherent activated T cell-mediated cytotoxicity against CMV-infected fibroblasts.
|
