| Project/Area Number |
06670789
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Osaka University |
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Principal Investigator |
YAMAMOTO Takehisa (1995) Faculty of Medicine, Osaka University, Department of Pediatrics, assistant professor, 医学部, 助手 (30231674)
山岡 完次 (1994) 大阪大学, 医学部, 助手 (60144490)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Sayuri Faculty of Medicine, Osaka University, Department of Pediatrics, assistant profe, 医学部, 助手 (10281122)
SHIMA Masaaki Faculty of Medicine, Osaka University, Department of Pediatrics, assistant profe, 医学部, 助手 (10252660)
山本 威久 大阪大学, 医学部, 助手 (30231674)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | renal failure / growth retardation / osteoblast / osteoclast / PTH / vitamin D responsive element / renal osteodystrophy / nitric oxide |
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| Research Abstract |
We investigate the mechanism of growth failure in patients with chronic renal failure in terms of the osteoclast formation and osteoblast function by sera from renal failure patients. Experiment 1 : Effect of sera from renal failure patients on the osteoclast-like cells formation. The sera were obtained from the renal failure patients on hemodialysis. The PTH levels in three patients are within normal range. The addition of sera from renal failure patients (20% CM) with alphaMEM significantly stimulated the osteoclast-like cells formation. These effect was abolished in the presence of massive larginine in the same experiment. These data suggest that the substance to inhibit nitric oxide synthetase might be involved in the phenomena of the increased osteoclast-likd cells formation in number. Experiment 2 : Effect of sera form renal failure patients on the 1,25 (OH)_2D_3 action in mice osteoblastic cell line (MC3T3-E1). The effect of sera on the rat osteocalcin gene induction by 1,25 (OH)_2D_3 was assessed. Rat osteocalcin VDRE was transfected by the calcium-phosphate precipitation method. The addition of sera from renal failure patients (10% CM) or 10^<-4>M PTH with alphaMEM had no effect on the osteocalcin gene induction. These data indicated that the sera from the renal failure patients had no influence on the osteoblastic cells by this method. However, these results does not exclude the possibility of the effect of renal failure sera on the osteoblastic cells in another method. Further study is necessary to elucidate the truth.
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