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Stady on haman skin-derived IL-7

Research Project

Project/Area Number 06670849
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionHokkaido University

Principal Investigator

MATSUE Hiroyuki  Hokkaido Univ. School of Medicine Instructor, 医学部, 助手 (10250424)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsIL-7 / Keratinocyte / CTCL / IL-7レセプター / 菌状息肉症
Research Abstract

1. The expression of IL-7 receptor on the infiltrated cells of CTCL :
IL-7 receptor-positive lymphocytes among the infiltrated cells of CTCL were stained with anti-IL-7 receptor antibody stronger than those in the inflammatory skin disease such as atopic dermatitis and psoriasis vulgaris.
2. IL-7 levels in the serum of patients with CTCL :
The serum IL-7 levels of patients with various skin diseases are much higher than those of normal individuals. Normal individuals (5 people) ; 5.2pg/ml, MF (6) ; 10.5, ATL (2) ; 7.1, CTCL (2) ; 20.5, Atopic dermatitis ; 16.7.
3.and 4. Establishment of Mycosis cell lines :
The infiltrated lymphocytes from the skin lesions of MF were cultured with IL-2 and IL-7. Several cell lines were established. Phenotype analysis of all cell lines revealed CD4 negative and CD8 positive cells.
5. Expression of IL-7 and its receptor in keratinocytes :
In RT-PCR analysis, IL-7 and its receptor are constitutively expressed on human primary cultured keratinocytes. Supernatants of cultured keratinocytes contained 5 pg/ml IL-7, assessed by ELISA.
6. Effects of IL-7 on keratinoctes :
In preliminary experiments, IL-7 did not affect growth and survival of keratinocytes. In order to find out effects of IL-7 on keratinocytes, we are now conducting experiments looking at effects of IL-7 on keratinocyte-derived cytokine expression.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] H. Matsue: "Keratinocytes constitutively express the Fas antigen that mediates apoptosis in IFN-α-treated cultuved keratinocytes" Arch. Dermatol. Res.287. 315-320 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Alexander Kaplan: "The effects of cyclosporin A and FK506 on proliferation and IL-8 production of cultured human keratinocytes" Journal of Dermatological Science. 10. 130-138 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Yokota: "Identification of mRNA-rich keratinocytes in the basal/suprabasal layers in psoriatic skin" (submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Matsue, H.Kobayashi, T.Hosokawa, T.Akitaya, A Ohkawara.: ""Keratinocytes constitutively express the Fas antigen that mediates apoptosis in INFgamma-treated cultured keratinocytes"" Arch. Dermatol. Res.287. 315-320 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Alexander Kaplan, Hiroyuki Matsue, Akihiko Shibaki, Toshimitsu Kawashima, Hitoshi Kobayashi, Akira Ohkawara: ""The effects of cyclosprin A and FK 506 on proliferation and IL-8 production of cultured human keratinocytes"" Journal of Dermatological Science. 10. 130-138 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Koichi Yokota, H.Matsue, A.Shibaki, T.Kawashima, H.Kobayashi, A.Ohkawara: ""Identification of mRNA-rich keratinocytes in the basal/suprabasal layrs in psoriatic skin"" (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Matsue: "Keratinocytes constitutively express the Fas antigen that mediates apoptosis in IFNγ-treated cultured keratinocytes" Arch.Dermatol.Res.287. 315-320 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Alexander Kapolan: "The effects of cyclosporin A and FK506 on proliferation and IL-8 prouduction of cultured human keratinocytes" Journal of Dermatological Science. 10. 130-138 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Kaplan: "The effects of cyclosporin A and FK 506 on proliferation and IL-8 production of cultured human Keratinocytes" Journal of Dermatological Science. (in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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