| Project/Area Number |
06670852
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
KATO Taizo Tohoku University SCHOOL,MEDICAL of DERMATOLOGY,ASSOCIATE PROFESSOR, 医学部, 助教授 (20004898)
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| Co-Investigator(Kenkyū-buntansha) |
TERUI Tadashi Tohoku University SCHOOL,MEDICAL OF DERMATOLOGY,ASSISTANT PROFESSOR, 医学部附属病院, 講師 (30172109)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | epidermal corneocytes / neutrophils / Staphylococcus / chemiluminescence / iC3b / CR3 / iC3b / 表皮角層 / 補体 |
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| Research Abstract |
We quantitatively assessed neutrophil adhesion to the serum-treated epidermal corneocyte sheet, which was mediated by activation of the alternative complement pathway. Addition of either anti-CD 18 or anti-CD11b antibody to the assay system resulted in a marked reduction of neutrophil adhesion. We also demonstrated that iC3b was formed on the serum-treated corneocytes. These findings indicate neutrophil attach to serum-treated corneocytes through an interaction of CR3 expressed on neutrophils with iC3b-coated corneocytes. Homogenized corneocytes suspension pretreated with Staphylococcus auresu for 60 min at 37ﾟCinduced an intense respiratory burst of neutrophils. Its peak chemiluminescence was about 3 times higher than those stimulated with corenocytes or St. aureus alone. This finding sugggests that the interaction between corneocytes and neutrophils may be a unique biodefense mechanism against microbes.
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