| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
To investigate the mechanism of morphological preservation of radiation therapy (RT), clinical, pathological, and molecular biological research was undertaken. By photographical and radilogical comparison between the pre-RT morphology and post-RT morphology, it was conclusive that morphological preservation is not merely ordinal tissue repair, but rather complex regeneration. Immunostaning of E-cadherin, which is one of the cell-cell adhesion molecule important in morphological developmement, showed that E-cadherin is stained even 5 years after the 65 Gy irradiation to the larynxgeal surface. Cell migration assay showed that more number of epitherial cells, HSC3 and CA9-22, were migrated by the conditioned medium of MRC5 which was irradiated 10 Gy than that of non-irradiated MRC5 Immunobiochemical quantitative analysis showed that the conditioned medium of MRC5 has more amount of HGF after 10 Gy irradiation than that of non-irradiated MRC5. These resultus suggested that morphological preservation after radiotherapy is depend on the molucular control of normal tissue, of which mechanism still await more investigation.
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