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Radiobiological factors determining the radiosensitivity of radioresistant cancer cells

Research Project

Project/Area Number 06670906
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Radiation science
Research InstitutionTokyo Medical and Dental University

Principal Investigator

YAMADA Ichiro  Tokyo Medical and Dental University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90182518)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsRadioresistance / Cancer Cells / Radiobiology / Melanoma Cells / Radiosensitivity / PLD Repair / Cell Kinetics
Research Abstract

In an attempt to elucidate the mechanism of radioresistance in melanoma cells, we demonstrated the following facts in the experimental system using human and murine melanoma cells : (1) the radiation dose-survival curve in melanoma cells had a larger shoulder portion in the low dose region, showing a marked radioresistance to doses commonly used in the conventional fractionated radiotherapy ; (2) melanoma cells showed a prominent repair of potentially lethal damage (PLD) after irradiation ; (3) melanoma cells had a relatively high fraction of S-phase cells which were more radioresistant than other phase cells in the cell cycle ; and, (4) pretreatment of melanoma cells with L-dopa significantly reduced the capacity for sublethal radiation damage in melanoma cells.
On the basis of these results, we have studied the cell kinetics, melanin production, GSH metabolism, and DNA repair, so as to further elucidate the mechanism of radioresistance of melanoma cells. All of melanin-producing melanoma cell lines showede a larger shoulder portion in the low dose region and a larger repair of PLD in various conditioned examined. The synchronized culture method demonstrated the change of radiosensitivity during the cell cycle and the survival curves of cells in each phase of the cell cycle. GSH levels and GSH-related enzymes activity were examined, suggesting the relationship between the GSH metabolism and radiosensitivity. Further, unscheduled DNA synthesis (UDS) after irradiation were also examined, so as to elucidate the relationship between the DNA repair and radiosensitivity.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Ichiro Yamada: "SPECT and MR imaging evaluations of the posterior circulation in moyamoya disease" J Nucl Med. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "Posterior circulation in moyamoya disease:angiographic study" Radiology. 197. 239-246 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "Moyamoya disease:comparison of assessment with MR angiography and MR imaging versus conventional angiography" Radiology. 196. 211-218 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "Takayasu arteritis:evaluation with MR imaging" Radiology. 188. 89-94 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "Moyamoya disease:diagnosis with three-dimensional time-of-flight MR angiography" Radiology. 184. 773-778 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "The killing effect of 4-S-cysteaminylphenol,a newly synthesised melanin precursor,on B16 melanoma cells lines" Br J Cancer. 63. 187-190 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "Year Book of Neuroradiology" Grossman,R.,ed.,Mosby Inc.,Chicago(in press), (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "Year Book of Rheumatology" Sergent,J.S.,ed.,Mosby Inc.,Chicago, 387 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Murata, Y., Umehara, I., Suzuki, S., Matsushima, Y: "SPECT and MR imaging evaluations of the posterior circulation in moyamoya disease" J.Nucl.Med.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki, S., Yamada, I., Himeno, Y: "Angiographic findings in Buerger disease" Internat.J.Cardiol. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Murata, Y., Yamada, I., Sumiya, Y., Shichijo, Y., Suzuki, Y: "Abdominal macronodular tuberculomas : MR imaging findings" J.Comput.Assist.Tomogr.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Murata, Y., Yamada, I., Sumiya, Y., Shichijo, Y.: "Magnetic resonance imaging findings in three patients with abdominal tuberculosis" Fortschr.Roentgenstr.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Himeno, Y., Suzuki, S., Matsushima, Y.: "Posterior circulation in moyamoya disease : angiographic study" Radiology. 197. 239-246 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Suzuki, S., Matsushima, Y: "Moyamoya disease : comparison of assessment with MR angiography and MR imaging versus conventional angiography" Radiology. 196. 211-218 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Suzuki, S., Matsushima, Y: "Moyamoya disease : diagnostic accuracy of MRI" Neuroradiology. 37. 356-361 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Murata, Y., Yamada, I., Isotani, E., Suzuki, S: "MRI in spontaneous cerebrospinal fluid rhinorrhoea" Neuroradiology. 37. 453-455 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Numano, F., Suzuki, S: "Takayasu arteritis : evaluation with MR imaging" Radiology. 188. 89-94 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Suzuki, S: "Primary uterine lymphoma : MR imaging" Amer.J.Roentgenol. 160. 662-663 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Seki, S., Ito, S., Suzuki, S., Matsubara, O., Kasuga, T: "The killing effect of 4-S-cysteaminylphenol, a newly synthesised melanin precursor, on B16 melanoma cell lines" Br.J.Cancer. 63. 187-190 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Suzuki, S., Matsushima, Y: "Moyamoya disease : comparison of assessment with MR angiography and MR imaging versus conventional angiography" Year Book of Neuroradiology, Grossman, R., ed., Mosby-Year Book, Inc., Chicago. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, I., Numano, F., Suzuki, S: "Takayasu arteritis : evaluation with MR imaging" Year Book of Rheumatology, Sergent, J.S., ed., Mosby-Year Book, Inc., Chicago. 258-260 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ichiro Yamada: "SPECT and MR imaging evalutions of the posterior circulation in moyamoya disease" J Nucl Med(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "Posterior circulation in moyamoya disease:angiographic study" Radiology. 197. 239-246 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "Moyamoya disease:comparison of assessment with MR angiography and MRangiography and MRimageing versus conventional angiography" :Radiology. 196. 211-218 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "Takayasu arteritis:evaluation with MR imaging" Radiology. 188. 89-94 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "Moyamoya disease:diagnosis with three-dimensional time-of-flight MR angiography" Radiology. 184. 773-778 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "The killing effect of 4-S-cysteaminylphenol,a newly synthesised melanin precursor,on B16 melanoma cells lines" Br J Cancer. 63. 187-190 (1991)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "Year Book of Neuroradiology" Crossman,R.,ed.,Mosby Inc.,Chicago(in press), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "Year Book of Rheumatology" Sergent,J.S.,ed.,Mosby Inc.,Chicago(in press), (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ichiro Yamada: "Assessing moyamoya disease:comparative results of MR angiography and MR imaging versus conventional angiography as diagnostic tools" Radiology. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Yamada: "Moyamoya disease:diagnostic accuracy of MR imaging" Neuroradiology. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Yamada: "Takayasu arteritis:evaluation with MR imaging" Radiology. 188. 89-94 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Yamada: "Moyamoya disease:diagnosis with three-dimensional time-of-flight MR angiography" Radiology. 184. 773-778 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Yamada: "The killing effect of 4-S-cysteaminylphenol,a newly synthesised melanin precursor,on B16 melanoma cell lines" Br.J.Cancer. 63. 187-190 (1991)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Yamada: "The effect of L-dopa on the potentiation of radiation damage to human melanoma cells" Br.J.Cancer. 62. 33-36 (1990)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Yamada: "Year Book of Rheumatology" Sergent,J.S.,ed.,Mosby Inc.,Chicago(in press),

    • Related Report
      1994 Annual Research Report
  • [Publications] 山田一郎: "全身のMRI" 多田信平編集、金原出版、東京, 340 (1992)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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