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Molecular Biological Study on the Role of Heparic Lipase on Arherogenesis

Research Project

Project/Area Number 06671027
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionOsaka University

Principal Investigator

NOZAKI Shuichi  Osaka Univ.Med School Ass.Prof., 医学部, 助手 (30252646)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Tadashi  Osaka Univ.Med School Ass.Prof., 医学部, 助手 (90252668)
TAKEMURA Kaoru  Osaka Univ.Med School Assoc.Prof., 医学部, 講師 (00161240)
MATSUZAWA Yuji  Osaka Univ.Med School Prof., 医学部, 教授 (70116101)
平野 賢一  大阪大学, 医学部附属病院, 医員
毛受 正和  大阪大学, 医学部附属病院, 医員
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordshepatic lipase / remnant / HDL / atherosclerosis / リポフェクション
Research Abstract

In our previous reports, we clarified that the activity of hepatic lipase is inversely correlated with the levels of IDL-cholesterol in primary hyperlipidemic subjects and hypothyroidism, suggesting that hepatic lipase plays an important role on remnant metabolism. We further reported that low hepatic lipase activities were considered to be one of the major causes of high HDL cholesterol level in some type of hyperHDL-cholesterolemia We found that the dyslipidemia in primary biliary cirrhosis (PBC) is partly due to the low activity of hepatic lipase. In the present study, we report that the combined deficiency of hepatic and CETP is a risk for coronary artery disease in part due to reduced reverse cholesterol transport. These data suggested that increase of reverse cholesterol transport by hepatic lipase and CETP will facilitate cholesterol effulux from foam cell and prevent foam cell formation.In this study, based on these observations, we hyppothesized that the increase of hepatic lipase in vivo will decrease the extent of forming foam cells by increasing cholesterol reverse transport and decreasing the levels of remnant lipoproteins in plasma. We first made HVJ-liposome by incorpoating the gene with HVJ into liposome. The HVJ-liposome was injected into portal vein of rats and the expression of the gene in liver was investigated. We found that the expression in liver by the HVJ-liposome method was very low. We are now trying to establish the innovated method for the HVJ-liposome for establishing more highly expressing system in liver.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kenich Hirano: "Atherosclerotic disease in marlted hyperalphal: poproleinemia Combined reduction of cholesteryl ester transfer piotein and hepatic triglyceride lipase" Arterioscler Thromib Vas Biol. 15. 1849-1856 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野崎秀一: "甲状腺機能低下症のリポ蛋白質異常とそのメカニズム-肝性リパーゼを中心として" 医学のあゆみ. 168. 932-936 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野崎秀一: "肝性リパーゼの生理的意義の研究-抗高脂血症薬剤と肝性リパーゼ活性" Therapeutic Research. 15. 878-883 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野崎秀一: "肝性リパーゼ (HTGL)" 現代医療. 28. 47-50 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野崎秀一: "高トリグハセライド血症とレムナントリポ蛋白" Pharma Medica. 14. 81-87 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野崎秀一: "肝性リパーゼの生理的意義の研究" Therapeutic Research. 17. 451-460 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirano K,Yamahsita S,Nozaki S,et al.: "Atherosclerotic disease in marked hyperalphalipoproteinemia : combined reduction of cholesteryl ester transfer protein and hepatic triglyceride lipase" Arrterioscler Thromb Vasc Biol. 15. 1849-1856 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nozaki S: "Dyslipidemia in hypothyroidism and its mechanism-the role of hepatic lipase" Igaku no Ayumi (in Japanese). 168. 932-936 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nozaki S,and Matsuzawa Y: "Hypertriglyceridemia and remnant lipoprotein" Pharma Medica (in Japanese). 14. 81-87 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nozaki S,and Matsuzawa Y.: "Effects of bezafibrate, probucol and niceritrol on lipoprotein fractions and hepatic triglyceride lipase activity in hyperlipidemic patients" Therapeutic Research (in Japanese). 15. 878-883 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nozaki S.: "Hepatic lipase" Gendai lryo (in Japanese). 28. 47-50 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nozaki S,and Matsuzawa Y.: "Study on the role of hepatic lipase" Therapeutic Research (in Japanese). 17. 451-460 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeshi Arai: "Marked increase of plasma cholesteryl ester tnansfer protein in obese subjects-corralation between plasma CETP actiulty,highdemsity lipoprotein cheleaterol levels and body dot distrihuner" Arterioscl.Thromb.14. 1129-1136 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yuya Ueyama: "Familial hypercholesterulaeinia-like sundwnme with apolipoprotein E-7 associated with marked Achilles tendor xanthomas and coronang artery discase:a report of two cases" J.Jntern.Med.235. 169-174 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutomu Nakagawa: "Marked hypocholesterolemia in a case with adrenal adenoms-Enhanced catabolism of lowdensity lipoprotein(LDL)via the LDL receptors of famor cells" J.Clino.Endocrinol.Merab.80. 92-96 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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