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Basic study for genetic therapy by analysis of intracellular signal transduction in thyroid carcinoma cell

Research Project

Project/Area Number 06671039
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionNAGASAKI UNIVERSITY

Principal Investigator

NAMBA Hiroyuki  NAGASAKI UNIVERSITY,SCHOOL OF MEDICINE,ATOMIC DISEASE INSTITUTION,ASSOCIATE PROFESSOR, 医学部・原研発症予防部門, 助教授 (80237635)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Shunichi  NAGASAKI UNIVERSITY,SCHOOL OF MEDICINE,ATOMIC DISEASE INSTITUTION,PROFESSOR, 医学部・原研発症予防部門, 教授 (30200679)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsTHYROID / RADIATION / CANCER / IODINE / 甲状腺がん / 細胞周期 / アポトーシス / 癌遺伝子 / 分子生物学
Research Abstract

1) Previous epidemiology study shows thyroid tissue is prone to radiation induced tumorigenesis. To investigate whether the tissue specific intracellular responses to irradiation in thyroid cells is existed or not, we have done the several studies. To this purpose, we used primary cultured thyroid cell that has normal genetic background, and some cell lines established from thyroid carcinoma that have genetic defects, for instance, p53 mutation. The survival rates after 2 Gy irradiation were 18% and >58% in primary thyroid cell and thyroid carcinoma cell line, respectively. G1 arrest after irradiation has been shown only in primary thyroid cell but not in thyroid carcinoma cells. It suggests G1arrest is depend on p53 status. We can find apoptosis is rare event in thyroid cells after radiation exposure. Next, we checked the change of the expression of the p53 down stream genes (p21/WAF1, Bax and FAS/APO1) after radiation. Although the expression of p21/WAF1 gene was increased, no change of expression was observed in Bax and FAS/APO gene. These results suggest there is thyroid tissue specific response after irradiation. We published these data on Cancer Res.
2) We developed semi-automated measurement apparatus of urinary iodine. We published these data on Clinical Chemistry.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Namba,H: "Radiation-induced G1 arrest is selectively mediated by the p53-WAF1/Cipl pathway in human thyroid cells." Cancer Res.55. 2075-2080 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Maeda,H: "A single missense mutation in codon 918 of the RET proto-oncogene in sporadic medullary thyroid carcinomas" Endocrine J.42. 245-250 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hosoda,C.: "Establishment and characterization of a malignant epitheloid hmangioendothelioma from mouse thyroid tumor." Endocrine J.42. 341-350 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuo,T: "Inhibition of epidermal growth factor binding system by ionizing radiation in A431 human squamous carcinoma cells." Cancer Lett.89. 153-159 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda K: "Automated measurement of urinary iodine with use of ultraviolet irradiation." Clin.Chem.41. 581-585 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito,M: "Childhood thyroid disease around Chernobyl evaluated by ultrasound examination and fine needle aspiration cytology." Thyroid. 5. 365-368 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Namba, H et.al.: "Radiation-induced G1 arrest is selectively mediated by the p53-WAF1/Cip1 pathway in human thyroid cells." Cancer Res.55. 2075-2080 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Maeda, H et.al.: "A single missense mutation in codon 918 of the RET proto-oncogene in sporadic medullary thyroid carcinomas" Endocrine J.42. 245-250 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hosoda C et.al.: "Establishment and characterization of a malignant epitheloid hmangioendothelioma from mouse thyroid tumor." Endocrine J.42. 341-350 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuo, T et.al.: "Inhibition of epidermal growth factor binding system by ionizing radiation in A431 human squamous carcinoma cells." Cancer Lett.89. 153-159 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuda K et.al.: "Automated measurement of urinary iodine with use of ultraviolet irradiation." Clin.Chem.41. 581-585 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito, M,et.al.: "Childhood thyroid disease around Chernobyl evaluated by ultrasound examination and fine needle aspiration cytology." Thyroid. 5. 365-368 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hosoda C et al: "Establishment and characterization of a malignant epitheloid hemangioendothelioma from mouse thyroid tumor." Endocrine J. 42. 341-350 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Maeda S et al: "A single missense mutation in codon 918 of the RET proto-oncogene in sporadic medulllary thyroid carcinomas." Endocrine J. 42. 245-250 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Namba H et al: "Radiation-induced G1 arrest is selectively mediated by the p53-WAF1/Cip1 pathway in guman thyroid cells." Cancer Res. 55. 2075-2080 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matuso T et al: "Inhibition of epidermal growth factor binding system by ionizing radiation in A431 human squamous carcinoma cells." Cancer Lett. 89. 153-159 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuda K et al: "Automated measurement of urinary iodine with use of ultraviolet irradiation." Clin Chem. 41. 581-585 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsumoto T: "Effects of IL-1 β on insulin-like growth factor-1 autocrine/paracrine axis in cultured rat articular chondrocyte." Ann Rheum Dis. 153. 128-133 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ito M: "Expression of parathyroid hormone-related peptide in relation to perturbations of gastric motility in the rat." Endocrinology. 134. 1936-1942 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akita S: "Thyroid hormone action of rat calvarial sutures." Thyroid. 4. 99-106 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeshita A: "Sequence analysis of the thyrotropin (TSH)receptor gene in congenital primary hypothyrodism associated with TSH unresponsiveness." Thyroid. vol. 255-259 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Usa T: "Correlation between supression of c-myc and antiproliferative effect of transforming growth factor β1 in the thytoid carcinoma cell growth." Endocrinology. 135. 1378-1384 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamashita S: "A report on the 1994 Chernobyl Sasakawa Project Workshop." Sasakawa Memorial Health Foundation, 322 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamashita S: "Chernobyl Sasakawa Health and Medical Cooperation-1994.Nagasaki Symposiumon Chernobyl: Update and Future.Nagataki Sed" Elsevier Science, 272 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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