Molecular biology of 3q27 translocation in lymphoid malignancies

• Project/Area Number: 06671110
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Dokkyo University School of Medicine
• Principal Investigator: NAKAMURA Yuichi Dokkyo University School of Medicine, Department of Medicine, Lecturer, 医学部, 講師 (20227896)
• Project Period (FY): 1994 – 1995
• Project Status: Completed (Fiscal Year 1995)
• Budget Amount: ¥1,500,000 (Direct Cost: ¥1,500,000); Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: Malignant lymphoma / Chromosomal translocation / BCL6 gene / リンパ系腫瘍 / 3q27転座 / BCL-6遺伝子 / 遺伝子再構成

Research Abstract

First, we studied the structural alterations within the 5' part of the BCL6 gene on the band 3q27, where is frequently affected by chromosomal translocations in B cell non-Hodgkin's lymphoma (B-NHL) patients. We found that five out of 11 patients with BCL6 abnormality had two independent DNA rearrangements on both alleles, presenting multiple rearranged bands on Southern blotting.
Furthermore, in one of them, it was revealed that an internal DNA deletion of 1.5kb area was generated within the BCL6 locus on untranslocated chromosome 3. Our results suggest that the occurrence of biallelic rearrangements and submicroscopic local recombinations would be unique features with BCL6 abnormality in B-NHL.Next, we analyzed aberrant expressions of the BCL6 mRNA recognized in two cell lines harboring 3q27 translocations.
Analysis of cDNA from these cells revealed that the 5' non-coding region of the BCL6 mRNA was substituted by transcripts from immunoglobulin genes including intronic sequences, forming unique chimeric transcripts.

Research Products

• [Publications] Yuichi Nakanura,et al.: "Biallelic DNA rearrangements and deletions within the BCL-6 gene in Bcell non-Hodgkin's Iymphoma." British Journal of Haematology. 90. 404-408 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yuichi Nakamura et al.: "Internal DNA deletion within the BCL-6 gene on untranslocated chromosome in non-Hodgkin's lymphoma with 3q27 abnormality." Leukemia. 10(in press). (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakamura Y,Miki T,Kawamata N,Hirosawa S,Saito K,Enokihara H,Furusawa S,Shishido H.: "Biallelic DNA rearrangements and deletions within the within the BCL-6 gene in B cell non-Hodgkin's lymphoma." British Journal of Haematology. 90. 404-408 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamura Y,Miki T,Miura I,Hashimoto K,Miura A,Akimoto K,Hirosawa S,Saito K, Enokihara H,Furusawa S,Shishido H.: "Internal DNA deletion within the BCL-6 gene on untranslocated chromosome in non-Hodgkin's lymphoma with 3q27 abnormality." Leukemia. (in press). (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamura Y, et al.: "Biollelic DNA rearrangements and deletions within the BCL-6 geme in Bcell non-Hodgkin's lymphoma." British Journal of Haemetology. 90. 404-408 (1995)
• [Publications] Nakamura Y, et al.: "Internal DNA deletion within the BCL-6 gene on untranslocated chromosome in non-Hodgkin's lymphoma with 3q27 abnormality" Leukemia. 10(印刷中). (1996)
• [Publications] Nakamura,Y.,et al: "Biallelic DNA rearrangements and deletions within the BCL-6 gene in Bcell NHL." British Journal of Haematology. (in press). (1995)