• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structure and function of ABO blood group antigens fond in human von Willebrand factor

Research Project

Project/Area Number 06671118
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionInstitute for Comprehensive Medical Science, Fujita Health University

Principal Investigator

MATSUI Taei  Inst.Comprehens.Med.Sci., Fujita Health University Research associate, 総合医科学研究所, 助手 (90183946)

Co-Investigator(Kenkyū-buntansha) TITANI Koiti  Inst.Comprehens.Med.Sci., Fujita Health Unviersity Professor, 総合医科学研究所, 教授 (60179942)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsABO Blood group / von Willebrand factor / FVIII Concentrate / Lectin / Protease / Bone marrow transplantation / PCR / Plasma glycoprotein / フォン・ビルブラント因子 / ELISA / 凝固因子
Research Abstract

We have found that human von Willebrand factor (vWF) has a unique characteristic of possessing covalently-linked ABO blood group antigens. We are interested in the physiological significance of these antigens in vWF.ABO blood group genotype in Japanese was determined by PCR and the relationship among the blood group genotype, plasma concentration and the ristocetin- and/or botrocetin-induced platelet agglutinating activity of vWF was surveyed. vWF concentration in plasma was only significantly lower in hetero (AO,BO) than homo (AA,BB), suggesting the presence of some correlation between the turnover ratio of vWF and the expression of H (O) substance on vWF.We surveyed blood group antigens in commercially available FVIII concentrates and found that only vWF and factor VIII shows antigenicity. We also found that IgM and IgG are associated with vWF,suggesting that naturally occurring antibodies against blood group antigen might be bound to vWF since these concentrates were prepared from pooled plasma. Plasma with each blood group may provide more efficient recovery of uniform vWF and FVIII.To isolate vWF of certain blood group from the concentrates, we examined several blood group specific lectins. HPA from Helix pomatia and UEA from Ulex europaeus recognized blood group A and H (O) substances in vWF,respectively. Other lectins were not sufficiently specific, though they show blood group specific hemagglutination. vWF rich in blood group A substance was separated from the FVIII concentrates by a HPA-agarose column. Using blood group specific vWF obtained by this lectin column, protease susceptibility of vWF is now being studied to elucidate the physiological funciton of blood group substances in vWF.Further, we are collecting data on the blood group expression of vWF in individuals with several blood group subtypes and with bone marrow transplantation between different blood groups.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Matsui, T. et al.: "Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin β-subunits." Biochemistry. 33. 14039-14048 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katayama, M. et al.: "Fibronectin and 130-kDa molecule complex mimics snake venom botrocetin-like structure potentially modulating association between von willebrand factor and vascular vessel wall." J. Biochem.117. 331-338 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shima, M. et al.: "ABO blood group genotype and plasma von willebrand factor in normal individuals." Vox Sang.68. 236-240 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ozeki, Y. et al.: "Tissue fibronectin is an endogenous ligand for galactin-1." Glycobiol.5. 255-261 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui, T. et al.: "Detection and isolation of plasm glycoproteins carring ABO blood group antigens by lectins(abstract)" Glycoconjugate J.12. 573 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 松井太衛: "von Willebrand因子の糖鎖の構造と機能" 日本血栓止血学会誌. 6. 421-432 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 松井太衛: "「Annual Review 血液 1995」" 中外医学社, 327(297-307) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui, T. & Titani, K.: "“Lectins in Medical Research"In Methods in Molecular Biology" Humana Press(in press), (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui, T.et al.: "Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin beta-subunits." Biochemistry. 33. 14039-14048 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katayama, M.et al.: "Fibronectin and 130-kDa molecule complex mimics snake venom botrocetin-like structure potentially modulating association between von Willebrand factor and vascular vessel wall" J.Biochem.117. 331-338 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shima, M.et al.: "ABO blood group genotype and plasma von Willebrand factor in normal individuals." Vox Sang.68. 236-240 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ozeki, Y.et al.: "Tissue fibronectin is an endogenous ligand for galactin-1." Glycobiol.5. 255-261 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui, T.et al.: "Detection and isolation of plasm glycoproteins carring ABO blood group antigens by lectins (abstract)" Glycoconjugate J.12. 573 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsui, T.& Titani, K.: ABO (H) blood group expression on the circulating glycoproteins. Humana Press, "Lectins in Medical Research" In Methods in Molecular Biology (in press), (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shima, M. et al.: "ABO blood group genotype and plasma von Willebrand factor in normal individuals." Vox Sang.68. 236-240 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ozeki, Y. et al.: "Tissue fibronectin is an endogenous ligand for galactin-1." Glycobiol.5. 255-261 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsui, T. et al.: "Detection and isolation of Plasma glycoproteins carring ABO blood group antigens by lectins" Glycoconjugate J.12. 573 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hamako, J. et al.: "Binding of human IgM from a rheumatoid factor to IgG of animal species." Comp. Biochem. Physiol.112B. 683-688 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松井太衛: "von Willebrand因子の糖鎖の構造と機能" 日本血栓止血学会誌. 6. 421-432 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsui, T. & Titani, K.: "ABO(H)blood group expression on tne circulating glycoproteins. "Lectins in Medical Research" In Methods in Molecular Biology" Rhodes, J. M. and Milton, J. D. eds. Humana Press(in press), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsui,T.et al.: "Purification and characterization of two Ca^<2+>-dependent lectins from coelomic plasma of seaCucumber,Stichopus japonicus" J.Biochem.116. 1127-1133 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsui,T.et al.: "Structural analysis of N-linked oligosaccharides of eguine chorionic qonadotropin and lutropin β-subunits." Biochemistry. 33. 14039-14048 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katayama,M.et al.: "Fibronectin and 130-kDa molecule complex mimics snake venom botrocetin-like structure potentially modulating association between von willebrand factor and vascular vessel wall." J.Biochem.117. 331-338 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ozeki,Y.et al.: "Developmental expression of D-galactoside binding lectin in sea urchin(Anthocidaris crassispina)eggs." Exp.Cell Res.216. 318-324 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shima,M.et al.: "ABO blood group genotype and plasma von Willebrand factor in normal individuals." Vox.Sang.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ozeki,Y.et al.: "Tissue fibronectin is an endogenous ligand for galectin-1." Glycobiology. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松井太衛: von Willebrand因子と血液型 「Annual Review血液1995」高久、宮崎、斎藤、溝口編、中外医学社, 327,269 (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi