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Molecular analysis of renal prostanoid receptors

Research Project

Project/Area Number 06671124
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Kidney internal medicine
Research InstitutionTohoku University

Principal Investigator

TAKEUCHI Kazuhisa  Tohoku Univ.2nd Dept Int Med, Assist Prof, 医学部, 助手 (40260426)

Co-Investigator(Kenkyū-buntansha) ABE Keichi  Tohoku Univ.2nd Dept Int Med, Prof, 医学部, 教授 (60004777)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsthromboxane-receptor / glameruli / renal distal tubules / TX receptor gene / EP3A receptor / cAMP / EP3B receptor / EP3 receptor gene / トロンボキサン / プロスタグランジン / 塩素イオン / 水腎症 / 受容体 / 染色体マッピング / プロスタグラジン / 腎機能 / 高血圧 / 腎炎 / クローニング / バイオテクノロジー
Research Abstract

1) We have cloned cDNA for rat thromboxane receptor, and using the cDNA we performed in situ hybridization histochemistry in the rat kidney. Rat TX receptor mRNA has been shown to be expressed exclusively in glomeruli. We moreover raised antibody against carboxyl-terminal tail of rat TX receptor, and using this antibody, immunohstochemistry was preformed. The immunohistochemistry has shown the possibility of TX receptor not only in glomeruli but also in renal distal tubules. TX receptor gene was assigned to 7q11. On the other hand, we cloned cDNA for rat TXsynthase, and the expression regulation in a rat kidney disease model is now under investigation.
2) Functional expression study in Cos-7 cells has suggested that EP3A receptor is linked to a signal transduction system leading to inhibition of cAMP formation, while EP3B receptor is linked with a signal transduction system leading to an increase in cytosolic free calcium concentration. These receptors may possibly exert their natriurtic and diuretic actions via their signal transduction systems. EP3 receptor gene was assigned to 2q44-45. On the other hand, we have cloned a chloride channel cDNA,and localized its mRNA along distal nephron segments where EP3 receptor is present.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Takeuchi K, Takahasi N, et al.: "Two isoforms of rat kdieny prostaglandin EP3 receptor: intrarenal expression co-localization" Biochem Biophys Res Commun. 199. 834-840 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi K, Yanai N, et al.: "Different cellular mechanisms of vasopressin receptor V1 and V2 subtype invasopressin-induced adenosine 3',5'-monophosphate formation in an ……" Biochem Biophys Res Commun. 202. 680-687 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi K, Takahashi N, et al.: "Functional difference between two isoforms of rat kidney prostaglandin receptor EP3 subtype" Biochem Biophy Res Commun. 203. 1897-1903 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe T, Takeuchi K, et al.: "Rat kidney thromboxane receptor:molecular cloning,signal transduction,and intrarenal expression localization" J Clin Invest. 96. 657-664 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takahashi N, Takeuchi K, et al.: "Immunohistochemical localization of rat thromboxane receptor and synthase in rat tesis." Endocrinology. 136. 4143-4146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takahashi N, Takeuchi K, et al.: "Immunohistochemical localization of thromboxane receptor and synthase in rat kidney" J Am Soc Nephrol. 6. 762- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi K,Takanashi N,et al: "Two isoforms rat kidney prostaglandin EP3 receptor : intrarenal expression colocalization" Biochem Biophys Res Commun. 199. 834-840 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi K,Yanai N,et al: "Different cellular mechanisms of vasopression receptor V1 and V2 subtype in vasopress in-induced adenosine 3', 5'-monophosphate formation in an immortalized renal tubule cell line TKC2" Biochem Biophys Res Commun. 202. 680-687 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi K,Takahashi N,et al: "Functional difference between two isoforms of rat kidney prostaglandin receptor EP3 subtype." Biochem Biophys Res Commun. 203. 1897-1903 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe T,Takeuchi K,et al: "Rat kdieny thromboxane receptor : molecular cloning, signal transduction, and intrarenal expression localization." J Clin Invest. 96. 657-664 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takahashi N,Takeuchi K,et al: "Immunohistochemical localization of rat thromboxane receptor and synthase in rat tesis" Endocrinology. 136. 4143-4146 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takahashi N,Takeuchi K,et al: "Immunohistochemical localization of thromboxane receptor and synthase in rat kideny." J Am Soc Nephrol. 6. 762 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeuchi K, Takahashi N, et al :"Functional analysis and chromosomal gene assignment of rat prostaglandin EP3 teceptor." Kidney Int.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takeuchi K, Nakagawara K, et al :"Assignment of the rat thromboxane receptor gene to 7q11 by in situ hybridization." Cytogenet. Cell Genet.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Abe T, Takeuchi K, et al :"Intrarenal expression of MDCK-type chloride channel." Nephron. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Abe T, Takeuchi K, et al. :"Rat kidney thromboxane receptor : molecular cloning, signal transduction, and intrarenal expression localization." J. Clin. Invest.96. 657-664 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi N, Takeuchi K, et al :"Immunohistochemcal localization of rat thromboxane receptor and synthase in rat tesis." Endocrinology,. 136. 4143-4146 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi N, Takeuchi K, et al :"Immunohistochemical localization of thronboxane receptor and synthase in rat kidney." J. Amer. Soc. Nephrol.6. 762- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takeuchi K.et al.: "Functional Difference befween two isoforms of rat kidney Prostoglandir EP_3 receptor" Biochem.Biophys.Res.Commun.203. 1897-1903 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeuchi.K.et al.: "Different cellular Mechanisms of Vasopressin v_1 and v_2 receptor subtype in vasopressin-induced cAMP from afion in TKC_2 cells" Biochem:Biophys.Res.Commun.202. 680-687 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeuchi K.et.al.: "Two isoforans of rat kidney prostoglaudin EP_3 recsptor" Biochem.Biophys.Res.Commun.199. 934-840 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeuchi K.et al.: "Molecular Cloning and Intrarenal Localizafion of the Rat Prostagludin EP_3 Receptor" Biochem.Biophys.Res.Commun.194. 885-891 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Abe T.et al.: "Molecutar Cloniy and Tissue Expression of the Rat MDCK-type Chloride Channel." Bichim.Biophys.Acta.1173. 353-356 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Abe T.et al.: "Rat Kidney Thromboxane Receptor:Molecutar Cloning and Expression" J.Amer.Soc.Nephrol.4. 481- (1993)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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