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Mechanism of inflammatory cell infiltration in the kidney tissue of glomerulonephritis and diabetic nephropathy. -Elucidation of the role of cell adhesion molecules and development of therapeutic drugs.-

Research Project

Project/Area Number 06671141
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Kidney internal medicine
Research InstitutionOkayama University

Principal Investigator

SHIKATA Kenichi  Okayama University Medical School, professor, 医学部, 助手 (00243452)

Co-Investigator(Kenkyū-buntansha) MAKINO Hirofumi  Okayama University Medical School, associate professor, 医学部, 助教授 (50165685)
小川 さえ子  岡山大学, 医学部・附属病院, 医員
久代 昌彦  岡山大学, 医学部・附属病院, 医員
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsadhesion molecule / kidney / glomerulonephritis / diabetes mellitus / ICAM-1 / selectins / leukocyte / 糸球体腎炎 / 細胞接着分子 / スルファチド / 糖尿病性腎症
Research Abstract

In STZ-induced diabetic rat, we investigated the macrophage infiltration into the glomeruli and expression of ICAM-1. After induction of the diabetes, ICAM-1 was upregulated in the glomeruli. In addition, intraglomerular macrophage infiltration was observed. By the normalization of the blood glucose levels by insulin injection, expression of ICAM-1 and macrophage infiltration were prevened. These results indicated that ICAM-1 may paly a pivotal role in the macrophage infiltration of the diabetic nephropathy.
We examined the expression of P-and E-selectins in the kidney tissues by immunohistochemistry. Normal kidney specimens and kidney biopsy specimens of glomerulonephritis and diabetic nephropathy patients were used. In normal kidneys, P-and E-selections were not detected. In lupus nephritis and diabetic nephropathy, P- and E-selections were expressed in the glomeruli and the intertubular vessels. Selections may be involved in the leukocyte infiltration into the kidney tissues in lupus nephritis and diabetic nephropathy.
By using the L-selectin-IgG chimeric molecule (LEC-IgG), the distribution of L-selectin ligands were investigated. L-selectin ligands were distributed on the distal tubular epithelium. After ligation of ureters of rats, L-selectin ligands were disappeared from the tubular cells and redistributed intertubular vessels. Tubular monocyte infiltraion was also observed. By th e injection of sulfatide and and anti-L-selectin antibody, monocyte infiltration was inhibited. Monocyte adhesive pathway via L-selectin may play a key role in the monocute infiltration into the kidney tissue in rat ureter ligation animal model.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 和田 淳: "Clitical role of intercellular adhesion molecule-I in nephrotoxic serum nephritis" Nephron. 73. 264-272 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 槇野 博史: "細胞接着分子" 腎と透析. 臨時増刊号. 198-203 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 槇野 博史: "腎疾患と接着分子" 医学のあゆみ. 174. 71-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 四方 賢一: "腎炎と接着分子" 病理と臨床. 13. 375-381 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 四方 賢一: "Distribution of extracellular matrix receptors in various forms of glomerulonephritis" Am J Kidney Dis. 25. 680-688 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wada J et al.: "Critical role of intercellular adhesion milecule-1 in nephrotoxic surum nephritis." Nephron. 73. 264-272 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Makino H.: "Cell adhesion molecules." Kidney and Dialysis Sup.198-203 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Makino H.: "Kidney diseases and adhesion molecules." Igaku no ayumi. 174. 71-76 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shikata K.: "Glomerulonephritis and adhesion molecules." Pathology and Clinical Medicine. 13. 375-381 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shikata K.et al.: "Distribution of extracellular matrix receptors in nvarious form of glomerulonephritis." Am.J.KidneyDis. 25. 680-688 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 和田淳: "Clitical role of intercellular adhesion molecule-1 in nephrotoxic serum nephritis" Nephron. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] 槇野博史: "細胞接着分子" 腎と透析. 臨時増刊号. 198-203 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 槇野博史: "腎疾患と接着分子" 医学のあゆみ. 174. 71-76 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 四方賢一: "腎炎と接着分子" 病理と臨床. 13. 375-381 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 四方賢一: "Distribution of extracellular matrix receptors in various forms of glomerulonephritis" Am J Kidney Dis. 25. 680-688 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 槙野博史: "細胞接着分子" 腎と透析. (臨時増刊). 198-203 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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