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An teratological experimental study of brain abnormalities induced by intrauterine infection with cytomegalovirus

Research Project

Project/Area Number 06671173
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Embryonic/Neonatal medicine
Research InstitutionHamamatsu University School of medicine

Principal Investigator

TSUTSUI Yoshihito  Hamamatsu University School of Medicine, Professor, 医学部, 教授 (50073135)

Co-Investigator(Kenkyū-buntansha) MASAGO Sonomi (AIBA Sono)  Hamamatsu Unversity Hospital, Senior resident, 医学部付属病院, 医員
真砂 園真  浜松医科大学, 医学部・附属病院, 医員
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordscytomegalovirus / viral teratology / brain anomalies / neuronal migration / brain development / 胎内感染 / 脳形成障害
Research Abstract

The susceptibility of mice at different developmental stages to a relatively low titer of cell culuture-passeged murine cytomegalovirus (MCMV) infection was compared in terms of the urinary excretion of MCMV examined by plaque assay and in terms of the distribution of viral infection, determned by immunohistochemistry, using antibodies specific to the early nuclear antigenof MCMV.Viral infection on day 8.5 of getation (E8.5) into the conceptus and intraperitoneal infection on day 15.5 of gestation (E15.5), postnatal day 2 (P2), postnatal day 11 (P11), and 30 days after birth (P30), respectively, were performed. Embryonal and perinatal mice were more susceptible to MCMV in terms of urinary excretion of the virus and the presence of viral antigen-positive cells in the brain, lungs, and kidneys. In the embryonal and perinatal infection, the viral antigen-positive cells in the neurons of the cerebral cortex and hippocampus were retained late after birth, even though the positive cells in the lungs and kidneys had disappeared. In the mice infected on E8.5, small clusters of viral antigen-positive cells were detected only in the cortex and hippo-campus late after birth, without the urinary excretion of virus. These results suggest that when mice are infected with MCMV at the embryonal and perinatal stages, elimination of the infected neurons is delayd compared with that of the other cells in the lungs and kidneys. These findings provide a model for the anyalysis of pathogenesis of the subclinical congenital CMV infection that manifested clinically late after birth in humans as brain disorders.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Tsutsui Y.,Kashiwai A.Kawamura N.,Kododa C.: "Miorophthalmia and Cere bral atroph induced in mouse embryos by ingeetion with murine cytomegolovirus in mid_8 esfa fion." Am.J.Pathol.143. 804-812 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsui Y.: "Developmental disorders of the mouse brain induced by murine cytomegalovirus:animal modelsfor congenital cytomegolovirus infection." Pathol.Internat.45. 91-102 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsui Y.,Kashiwai A.,Aiba,Masago S.,Kosugi I.: "Prolonged infection of mouse brain neurons with murine cytomegatovirus affer pre-and perinatal infection" Arch.V,rol.140. 1725-1736 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsui Y., Kashiwai A., Kawamura N., Kadota C.: "Microphthalmia and cerebral atroph induced in mouse embryos by ingection with murine cytomegalovirus in midgestation." Am.J.Pathol.143. 804-812 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsui Y.: "Developmental disorders of the mouse brain induced by murine cytomegalovirus : animal models for congenital cytomegalovirus infection." Pathol. Internat.45. 91-102 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsui Y., Kashiwai A., Kawamura N., Aiba-Masago S.and Kosugi I.: "Prolonged infection of mouse brain neurons with murine cytomegalovirus after pre- and perinatal infection." Arch. Virol.140. 1725-1736 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsutsui Y,et al.: "Microph thaluia and cerebral atrophy induced in mouse embryos. infection with murine cytomogalovivus in midgestafion" American Journal of Pathology. 143. 804-812 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsutsui,Y: "Developmental disorders of the mouse brain induced by murine cytomegalovirus" Pathology International. 45. 91-102 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsutsui,Y,et al: "Prolongecl infection of mouse brain neurons with murine cytomoga lovivue after pre-and perinatal injection"

    • Related Report
      1995 Annual Research Report
  • [Publications] 筒井祥博: "Developmental disorders of the mouse brain induced by murine cytomegalovirus:animal models for congenifal cytomegalovirus infection" Pathology International. 45. 91-102 (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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