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Analysis of Non-functional Extra cellular Fluid Space in the Ischemic Reperfusion Injury Model

Research Project

Project/Area Number 06671180
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionTohoku University

Principal Investigator

HIRAKAWA Hisashi  School of Medicine hospital The Second Dept.of Surgery, Research Associate, 医学部附属病院, 助手 (10250763)

Co-Investigator(Kenkyū-buntansha) KATO Hirotaka  School of Medicine hospital The Second Dept.of Surgery, Research Associate, 医学部附属病院, 助手 (00240656)
DOI Hirotaka  School of Medicine hospital The Second Dept.of Surgery, Research Associate, 医学部附属病院, 助手 (90188839)
OHKOCHI Nobuhiro  School of Medicine hospital The Second Dept.of Surgery, Lecturer, 医学部, 講師 (40213673)
SHINEHA Ryuzaburou  School of Medicine hospital The Second Dept.of Surgery, Lecturer, 医学部, 講師 (20192106)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsnon functional extracellular fluid / third space / ischemia-reperfusion model / サイトカイン / IL-8 / メチルブレドンゾロン(MP) / オートラジオグラフィー / 手術侵襲 / 非機能的細胞外液量
Research Abstract

Non-functional extra cellular fluid space is demonstrated by means of 14C insulin distribution and auto radiography in rat lower limb ischemic reperfusion model.
Rats were divided into three group ; group I : 6 hours laparotomy, group II : 6 hours ischemia and reperfusion, group III : 6 hours ischemia but small amount of methylprednisolone (20mg/kg) was administrated before reperfusion. In each group 14C insulin was injected intravenously after reperfusion and i hour later washed out by saline. 14 C inuline was counted and autoradiographed by bioimaging-analyzer (BAS-2000).
Serum IL-8 concentration was increased significantly at 1,3,6 hours in the ischemic rats comparing with control rats, but in group III,IL-8 was not increased.
Non-functional extra cellular fluid was increased in group II,but wast in group III.Non-functional extra cellular fluid was increased not only in ischemic lesions but also in non-ishemic lesions (such as liver, lung, pancreas, skin).
Auto-radiography demonstrated non-functional extra cellular fluid space clearly and visually, and small amount of steroid which was administrated before reperfusion reduced the space remarkably.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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