| Project/Area Number |
06671181
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Akita University |
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Principal Investigator |
SHIKAMA Toshio Akita Univ.2nd Dep.of Surg.assistant, 医学部, 助手 (40196366)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMIYA Yoshihiro Akita Univ.2nd Dep.of Surg.instructor, 医学部, 講師 (30239321)
KITAMURA Michihiko Akita Univ.2nd Dep.of Surg.associate prof., 医学部, 助教授 (10153131)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | esophageal cancer / endotoxin / 2', 7'-dichlorofluorescin diacetate / respiratory disorder / H_2O_2 production / エンドトキシン / 換気障害 / 過酸化水素 / DCFH‐DA / 生体顕微鏡 / non‐septic endotoxemia / エンドトキシン血症 / translocation / G因子活性化物質値 |
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| Research Abstract |
[Objective]
This study was conducted to clarify the mechanism of respiratory disorder in the postoperative state in patients with esophageal cancer.
[Background data]
Translocational endotoxemia has been demonstrated in the postoperative and postburnt state in animals. Endotoxin is known to lead to the clinical manifestation of respiratory disorder. But the occurrence of endotoxemia had not been confirmed in the early postoperative state in patients with esophageal cancer. Then, we hypothesized that H_2O_2 production during the operation leaded to the respiratory disorder.
[Methods]
We used the computer image-analyzing system and 2', 7'-dichlorofluorescin diacetate for the marker of H_2O_2 production in vivo.
[Results]
H_2O_2 production in the pulmonary microcirculation of the lt. pulmonary artery blocked rabbits was higher than those of the lt. main bronchus blocked rabbits and control rabbits at each time point. In high-power view, H_2O_2 was detected in granulocytes.
[Conclusions]
Insuficient pulmonary circulation due to operative procedure may cause the respiratory disorder in the early postoperative state in patients with esophageal cancer.
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