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STRATEGY TO CONTROL XENOGRAFT REJECTION,AND POTENTIAL BENEFITS TO APPLY MHC-DEFICIENT DONOR

Research Project

Project/Area Number 06671184
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionChiba University

Principal Investigator

OCHIAI Takenori  Chiba University, Department of Surgery, associate proffessor, 医学部, 助教授 (80114255)

Co-Investigator(Kenkyū-buntansha) KOMORI Akiyoshi  Chiba University Hospital, Instractor, 医学部・附属病院, 医員
ISONO Kaichi  Chiba University, Department of Surgery, proffessor, 医学部, 教授 (70009489)
GUNJI Yoshio  Chiba University, Department of Surgery, assistant, 医学部, 助手 (60241957)
NAKAJIMA Kazuaki  Chiba University, Department of Surgery, assistant, 医学部, 助手 (20261919)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Keywordsleukotrien B4 / MHC / xenograft / FK506
Research Abstract

Treatment with leukotrien B4 (LTB4) antagonist (50mg/kg) alone failed to prolong allograft survival, and combination of LTB4 antagonist and FK506 (0.32mg/kg) did not work in allograft model. Mouse skin graft on rats treated with LTB4 antagonist survived 11.5days, while xenograft on untreated rats were reiected in 8 days. Combination of LTB4 antagonist and suboptimal dose of FK brought 14.5 day-graft survival, while MST of FK alone group was 9.5 days. In the CML analysis, pig-effectors stimulated with B10.BR (H-2^k) killed B10.BR,C3H/HeJ (H-2^k) B10.A (4R) (sharing K^k with B10.BR) and C3H-KBR (sharing D^k with B10.BR) targets, respectively ; however killing activity was not shown on C57BL/10 (H-2^b) and C3H.SW(H-2^b) targets.
In conclusion, LTB4 antagonist improved mouse skin graft survival on rats. This finding may support that neutrophile play a role in xenogeneic immune responses. Pig effectors recognize mouse class-I antigens and show killing activities on mouse targes. It may be expected that deletion of MHC antigens in xenograft might provide elongation of xenograft survival through the suppression of cellular responses.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 中島 一彰: "ラット頚部異所性心臓移植-カフ法-" Organ Biology. 2. 61-66 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 坂本 薫: "異種移植免疫反応モデルとしての37℃ヒト全血によるミニブタ肝灌流の試み." Organ Biology. 1. 71-72 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima K,Ochiai T,Suzuki T,et al.: "In vitro analysis of xenogeneic cellular responses." Ishoku (Japanese J of Transplnt). 28. 150-155 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima K,Ochiai T,and Isono K: "Method of heterotopic cardiac transplantation in rat model, -cuff method-" Organ Biology (in Japanese). 2. 61-66 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakamoto K,Nakajima K,Kenmochi T,et al.: "Utilization of organ preservation machine as an ex vivo swine liver perfusion with human whole bloood at 37^0C" Organ Biology (in Japanese). 1. 71-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima K,Ochiai T,Suzuki T et al.: "T cell Restriction Patterns in Pig Bone Marrow Chimera." Transplant Proc. 28. 1244-1245 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中島一章: "ラット頚部異所性心臓移植-カフ法-" Organ Biology. 2. 61-66 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 坂本薫: "異種移植免疫反応モデルとしての37℃ヒト全血によるミニブタ肝灌流の試み" Organ Biology. 1. 71-72 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 中島 一彰: "ラット頚部異所性心臓移植-カフ法-" Organ Biology. 2. 61-66 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 坂本 薫: "異種移植免疫反応モデルとしての37℃ヒト全血によるミニブタ肝灌流の試み" Organ Biology. 1. 71-72 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中島 一彰: "異種間細胞性免疫反応の検討" 移植. 28. 150-155 (1993)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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