| Project/Area Number |
06671197
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
IMAI Tsuneo Nagoya Univ.School of Med.Assistant Professor, 医学部, 助手 (80252245)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Yoshiharu Nagoya Univ.School of Med.Associate Professor, 環境医学研究所, 助教授 (80174308)
FUNAHASHI Hiroomi Nagoya Univ.School of Med.Assistant Professor, 医学部, 講師 (50135357)
TAKAGI Hiroshi Nagoya Univ.School of Med.Professor, 医学部, 教授 (70154755)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Clonality / MAOA / heterozygosity / Endocrine neoplasia / microsatelite / monoclonal / polyclonal / PCR |
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| Research Abstract |
Analysis of Clonality in Endocrine Neoplasias-MAOA microsatelite methods using fluorescence primer
DNA was extracted from peripheral white blood cells in female patients of various kinds of diseases. Monoamine oxidase type A (MAOA) gene was amplified by polymerase chain reaction (PCR) using fluorescence labeled primers. The amplified products were denatured and electrophoresed in 6% polyacrylamide gel and the bands were detected by automatic sequencer and analyzed using GeneScan 672 software (Applied Biosystems). The examined patients were ; aldosterone producing adenoma : 14, Cushing's syndrome (adrenal adenoma) : 5, pheochromocytoma : 1, virilizing adrenal tumor : 1, thyroid neoplasms : 4. All 24 patients' DNA were subjected to the clonal analysis. The informed cases (heterozygous for MAOA gene) were 17 of 24, so that the heterozygosity was 67%.
Heterotransplantation of Human Parathyroid Glands into Nude Mice
Grafts prepared from hyperplasia, adenoma, and normal glands which were resected at operation were implanted in the gluteus muscle of the recipient mice. Graft function was evaluated by measuring human intact PTH concentrations in sera of the mice. Transplantation of parathyroid tissues resulted in an increase in PTH concentrations for 4 weeks, reaching a plateau thereafter. The level remained unchanged for 8 weeks. Serum PTH concentrations were directly correlated with the volume of implanted tissues. The response of grafted parathyroid tissue to a low calcium level in sera was also observed.
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