The effect of allopurinol on skeletal muscle I-R injury and purine metabolism.
| Project/Area Number |
06671228
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Keio University.department of Surgery |
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Principal Investigator |
ORII Masahiro Keio University, Surgery instructor, 医学部, 助手 (40129383)
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| Co-Investigator(Kenkyū-buntansha) |
AKIYAMA Yosinobu Keio University, Surgery, Instructor, 医学部, 助手 (50245502)
YAMAZAKI Masanori Keio University, Surgery, Instructor, 医学部, 助手 (40239950)
SHIRASUGI Nozomu Keio University, Surgery, Instructor, 医学部, 助手 (90226324)
ASAMI Atsunori Keio University, Surgery, Instructor, 医学部, 助手 (30212492)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | I-R injury (Ischemic-Reperfusion) / allopurinol / skeletal muscle / microdialysis / viability / Microdialysis |
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| Research Abstract |
1.In this study, we determined whether allopurinol really reaches to reperfused muscle and reduces I-R (ischemia reperfusion) injury by inhibiting xanthine oxidase or not.
Microdialysis method combined with HPLC (high performance liquid chromatography) were employed and purines, MDA (malondialdehyde), allopurinol in gracilis muscle were measured continuously in I-R injury model of canine gracilis muscle. The effect was compared between Group N (no treatment : n=8), Group P (pre ischemic treatment : n=8) and Group R (pre-reperfusion treatment : n=8). Allopurinol reduced the increase of xanthine, uric acid, MDA in the muscle and CPK in blood effluent from gracilis muscle after reperfusion. Tissue protecting effect of allopurinol was more effective in group R than in group P.
And we measured superoxide producting activity of neutrophils in venous blood by chemiluminescence. Then there was no significant change by allopurinol treatment and allopurinol did not act as a scavenger of super oxide.
These data show that allopurinol reduce I-R injury mainly by inhibiting xanthine oxidase not by inhibiting neutrophil induced superoxide.
2.Skeletal muscle viability can be indicated by TTC assay, established method. But this method is invasive because it needs tissue samples. We investigated microdialysis method, aless-invasive method, can indicate skeletal muscle viability or not by using 10 hr. ischemic model of rat hind limb. Microdialysis method could indicate severely ischemic skeletal muscle viability.
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Report
(3 results)
Research Products
(4 results)
