• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Specific chemothrapy for perineural invasion of biliary tract cancer with conjugate of anticancer drug and anti-neural cell adhesion molecule antibody

Research Project

Project/Area Number 06671240
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionAKITA UNIVERSITY

Principal Investigator

TANAKA Jun-ichi  Akita University, Surgery, Research associate, 医学部, 助手 (30171763)

Co-Investigator(Kenkyū-buntansha) SATO Yasuhiko  Akita University, Surgery, Research associate, 医学部, 助手 (80235407)
KOTANAGI Hitoshi  Akita University, Surgery, Research associate, 医学部, 助手 (00161935)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsbiliary tract cancer / perineural invasion / neural cell adhesion molecule / anticancer drug-monoclonal antibody conjugate
Research Abstract

Conjugate of chemotherapeutic drug (Mitomycin C,MMC) and monoclonal antibody, which was anti-Neural cell adhesion molecule (NCAM) antibody was prepared and evaluated anticancer activity and antibody activity of the conjugate in vitro. MMC and anti-NCAM antibody conjugate was prepared according to Suzuki et al with cyanogen bromide (BrCN) method.
The degree of substitution was determined spectrophotometrically by assuming that MMC has absorbance with lambdamax=363nm, E1%1cm=623 and IgG has absorbance with lambda max=280nm, E1%/1cm=13.5. The degree of substitution which was calculated as follows : degree of substitution=mol concentration of drug contained in conjugate/mol concentration of antibody contained in conjugate, was 3.5 (average).
A drug activity of conjugate, which was tested by MTT method of the growth inhibition with neuroblastoma cell line (IMR-32) was maintained 66 to 95% compared with same concentration of MMC solution.
Antibody activity of conjugate, which was examined by immunohistochemical staining with Avidin-Biotinylated enzyme Complex on NCAM positive bile duct cancer, showed the same level as that of antibody alone.
In conclusion, MMC could be linked to anti-NCAM monoclonal antibody by BrCN method. Since this conjugate has both activity of drug and antibody, it can provide an useful tool for specific targeting cancer chemotherapy.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Seki H, Tanaka J, Sato Y, Kato Y, et al: "Neural cell aolhesion molecule (NCAM) and perineural in rasion in bile duct cancer" Journal of Surgical Oncology. 53. 78-83 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki H, Koyama K, Tanaka J, Sato Y, et al: "Neural cell aolhesion molecule and perineural invasion in gallblaolder cancer" Journal of Surgical Oncology. 58. 97-100 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki H,Tanaka J,Sato Y,Kato Y,Umezawa A,Koyama K: "Neural cell adhesion molecule (NCAM) and perineural invasion in bile duct cancer" Journal of Surgical Oncology. 53. 78-83 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki H,Koyama K,Tanaka J,Sato Y,Umezawa A: "Neural cell adhesion molecule and perineural invasion in gallbladder cancer." Journal of Surgical Oncology. 58. 97-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi