| Project/Area Number |
06671249
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
YASUHARA Hiroshi (1995) UNIVERSITY OF TOKYO,SURGERY,ASSISTANT PROFESSOR, 医学部(病), 講師 (50251252)
北山 丈二 (1994) 東京大学, 医学部(病), 助手 (20251308)
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| Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Osamu UNIVERSITY OF TOKYO,SURGERY,ASSISTANT PROFESSOR, 医学部(病), 助手 (10261976)
SETO Yasuyuki UNIVERSITY OF TOKYO,SURGERY,ASSISTANT PROFESSOR, 医学部(病), 助手 (00260498)
NAGAWA Hirokazu UNIVERSITY OF TOKYO,SURGERY,ASSOCIATED PROFESSOR, 医学部(病), 助教授 (80228064)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | esophageal cancer / immunohistochemistry / EGFR / lymphnode metastasis / 転移 / 接着分子 |
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| Research Abstract |
BACKGROUND : Many studies have reported the increased expression of epidermal growth factor receptor (EGFR) in various human malignancies and its association with the biologic behavior of the tumors.
METHODS : We performed an immunohistochemical analysis of the EGFR in 217 cases of human esophageal squamous cell carcinoma, 161 lymph node metastases and 23 foci of squamous dysplasias.The findings were correlated with clinicopathologic features, including the clinical outcome.Southern blot analysis was performed in 42 cases for the detection of DNA amplification of the EGFR gene and subsequently was correlated with EGFR immunoreactivity.
RESULTS : Epidermal growth factor receptor overexpression was detected in 71% of primary tumors and 88% of lymph node metastases, as compared to nonpathologic adjacent esophageal epithelium.Statistically significant correlations were observed between EGFR overexpression and sex, age, histologic type, and the presence of invasion.Tumor staining was classified into two patterns, homogeneous and heterogeneous, based on the distribution of EGFR-positive cells.The immunostaining patterns of primary tumors had a statistically significant correlation with histologic type, the presence of adventitial invasion, histologic stage and lymph node metastasis.There was a tendency toward a worse prognosis for those patients with EGFR overexpression in the primary tumor.Greater than 90% of the foci of squamous dysplasia demonstrated homogeneous EGFR overexpression.DNA amplification of the EGFR was observed in 21% of primary tumors, and all demonstrated immunohistochemical overexpression.
CONCLUSIONS : Immunohistochemical overexpression of the EGFR,which was more frequent than EGFR DNA amplification, appears to play an important role in biologic behavior of human esophageal squamous cell carcinomas.
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