| Project/Area Number |
06671262
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
KAWAKAMI Kazuhiko HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,SECOND DEPARTMENT OF SURGERY,ASSISTANT, 医学部, 助手 (10252181)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAGAWA Atsuo HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,PHOTON MEDICAL RESEARCH CENTER,VISITING, 客員教授
BABA Shozo HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,SECOND DEPARTMENT OF SURGERY,PROFESSOR, 医学部, 教授 (40107818)
TERAKAWA Susumu HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,PHOTON MEDICAL RESEARCH CENTER,PROFESSOR, 光量子医学研究センター, 教授 (50014246)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | ONCOGENE / COLORECTAL CANCER / IN-SITU HIBRIDIZATION / BCL-2 / 癌抑制遺伝子 / 転移 / 浸潤 / ヒト大腸癌肝転移モデル / PCR / UPA |
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| Research Abstract |
Bcl is a gene which was supposed to have a relation to apoptosis. In this study, we planned to analyze the localization of bcl-2 mRNA in human colon cancer by in situ RT-PCR method. Lymphnodes contracted with B-cell lymphoma cell were used as a positive control. Bcl-2 positive cells were detected by immuno histochemical staining, but the mRNA of bcl-2 were not detected by in situ RT-PCR method. It was not improved by charging the duration time of proteinase K treatment. The occurance of some degeneration of mRNA was suspected.
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