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Study on the grade of malignancy and postoperative recurrence in hepatocellular carcinoma with special reference to molecular biological analysis

Research Project

Project/Area Number 06671266
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionMIE UNIVERSITY

Principal Investigator

YOKOI Hajime  Mie University, Faculty of medicine, University Hospital lecturer, 医学部, 講師 (60174843)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
KeywordsHepatocellular carcinoma / Molecular biology / Grade of malignancy / Postoperative recurrence / Loss of heterozygosity on chromosome / nm23H1 gene / Cell adhesion regulator gene / E-cadherin gene / E‐cadherin / 分子生物学的悪性度評価 / 癌転移抑制遺伝子 / 接着分子
Research Abstract

Using the molecular biological methods, we examined the loss of heterozygosity (LOH) on chromosomes 13q, 16q and 17p, which are frequently detected in human hepatocellular carcinoma (HCC). We also examined the gene expression of antimetastatic gene, nm23H1, adhesion molecules, cell adhesion regulator (CAR) and E-cadherin (EC) which are associated with cancer metastasis. Finally, we analyzed the relationships between these factors and clinicopathological features and DNA ploidy pattern. Our results were as follows ;
1.The frequency of LOH,especially 16q chromosome and reduced expression of nm23H1, CAR,EC gene were increased along with the progression of the Stage of HCC.
2.CAR and EC genes also existed on 16q chromosome and their frequency of reduced expression was higher in those tumors with LOH of 16q chromosome.
3.In tumors with 2 and more LOH,including 16q chromosome, and reduced expression of nm23H1, CAR,EC,the frequency of portal vein invasion, intrahepatic metasstasis, aneuploid, mu … More ltiple intrahepatic recurrence, and extrahepatic metastasis were high.
4.We were able to confirm the multicentric occurence of HCC by analysing the differences of LOH pattern of tumors of multinodular HCC and primary and recurrent tumors in the resected specimens of recurrent HCC.
Thsese investigations demonstrated that molecular biological analysis was very useful to evaluate the grade of malignancy and diagnose muticentric occurence in HCC.With polymerase chain reaction (PCR) method with microsatellite marker for LOH and reverse transcriptase PCR (RT-PCR) method for gene expression, we can analyze a very small amount of sample, such as those obtained by needle biopsy, and thus, try to evaluate the prognosis preoeratively. As these genetic alteration occures in the late stage of HCC,it is important to investigate the genetic alteration in the early stage. Thus we plan to study the LOH in the early stage to identify new tumor suppressor genes on these chromosomes, and the relationship with abnormal DNA repair genes, detected in those who had hereditary nonpolyposis colorectal cancer, which causes carcinogenesis and progression of HCC. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 横井 一: "若年者B型肝細胞癌の臨床病理学的検討-背景因子、腫瘍進展度,切除後の予後" 日本臨床. 53. 698-703 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Kankrada: "Surgical treatment of hepatocellular carcinoma" Cancer Chemotherapy and pharmacology. 33. 12-17 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 横井 一: "肝細胞癌悪性度の判定-分子生物学的立場からみた悪性度-" KARKINOS. 7. 241-249 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野口 孝: "特集/小肝細胞癌 外科的治療の問題点" 外科治療. 71. 78-84 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野口 孝: "肝癌の集学的治療の適応と選択" 外科治療. 36. 1533-1542 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshifumi KAWARADA: "Surgical treatment of hepatocellular carcinoma" Cancer chemotherapy and pharmacology. 33. 12-17 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi NOGUCHI: "Clinical significance and problems of surgical treatment for small hepatocellular carcinoma" SURGICAL THERAPY. 71 (in Japanese). 78-84 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hajime YOKOI: "Molecular biological evaluation for the degree of malignancy in hepatocellular carcinoma" KARKIOS. 7 (in Japanese). 241-249 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi NOGUCHI: "Indication and selection of multimodality therapy for hepatocellular carcinoma" Geka Shinryo. 36 (in Japanese). 1533-1542 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hajime YOKOI: "Clinicopathological studies on juvenile hepatocellular carcinoma with hepatitis B infection-Background factors, tumor spread and prognosis after hepatic resection" Nippon rinsho. 53 (in Japanese). 698-703 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 横井 一: "若年者B型肝細胞癌の臨床病理学的検討-背景因子,腫瘍進展度,切除後の予後" 日本臨床. 53. 698-703 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Kawarada: "Surgical treatment of hepatocellular carcinoma" Cancer Chemotherapy and pharmacology. 33. 12-17 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 横井 一: "肝細胞癌悪性度の判定-分子生物学的立場からみた悪性度-" KARKINOS. 7. 241-249 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野口 孝: "特集/小肝細胞癌 外科的治療の問題点" 外科治療. 71. 78-84 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野口 孝: "肝癌の集学的治療の適応と選択" 外科診療. 36. 1533-1542 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 横井 一: "肝細胞癌の基礎と臨床-最近の動向-肝細胞癌悪性度の判定-分子生物学的立場からみた悪性度" KARKINOS. 7. 241-249 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 野口 孝: "小肝細胞癌 外科治療と問題点" 外科治療. 71. 78-84 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 横井,一: "1)肝細胞癌(臨床病理学的並びに分子生物的検討と治療成績)肝臓。胆道。脾臓の外科-研究と臨床-,水本龍二編奢" 医学図書出版, 19 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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