Intra cellulan Ca^<2+> dynamics in acute pancratitis

• Project/Area Number: 06671267
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: OHSHIO Gakuji Kyoto University, Surgery Graduate of Medicine, 医学研究科, 助手 (80131100)
• Project Period (FY): 1994 – 1995
• Project Status: Completed (Fiscal Year 1995)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: Acute Pancreatitis / Calcium / Acinar Cells / CCK / Amylase / Lipase / pancreatic duct obstruction / CDE / 膵管閉塞 / 膵液 / 膵頭十二指腸切除術 / プロテインキナーゼC

Research Abstract

We investigated digestive enzyme release following a short-term pancreatic duct obstruction in rats. An in vivo experiment demonstrated that a 5-hour pancreatic duct obstruction reduced digestive enzyme releaase evoked both by endogenously released CCK due to pancreatico-biliary diversion and by exogenous administration of CCK-8. In vitro experiments also showed that pancreatic duct obstruction reduced the maximal CCX-8 evoked amylase secretion. Anylase secretion evoked by the calcium ionophore and phorbol nyristate acetate was also decreased. These data suggested that pancreatic duct obstruction probably interferes with the secretory process downsream of hormone receptor binding, intracellular C a release and protein kinase c activation. The in vitro anylase secretion analysis of the CDE pancreatitis demonstrated a poor response curve. The application of CCK-8 (10pM) evoked intracellular Ca oscillations. Periodicity and anplitude of oscillations in the CDE groups were not significantly different. These results suggest that other mechanisms subsequent to the onset of intracellular Ca release are likely to be involved in the inhibition of enzyme secretion.

Research Products

• [Publications] Ohshio G. et al.: "Panorcettc exocrine secretion in shot-term pamcreaticduat obstraction inducedacute pancreatitis in rats." Diyertion. 55. 200-207 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wang ZH et al.: "Ettc of the oraladministrrtin of sepinosat nesilatl on cerulcia in dunudacrtepanrlathtnis in rats." Arzneimitiel Forehung. Drug Research.45. 1082-1086 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Okada N. et al.: "Intra cellular A^+ dymamics and in vitro cecretory responsc in a anti pamcrlatrris in ouced by a cholinedificient etion ine saprlemeted dilt in mnce" Dig. es. tin. 56. 502-508 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wang ZH et al.: "Inoured pamoreatic metallothionein and ghtathin lives: protecting again st ceraleia and faurochola te induced a auth pinarehtihie in rats." Pancreas.(in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohshio G et al.: "pancreatic exocrine secretion in short-term pancreatic duct obstruction induced acute pancreatitis in rats." Digertiln. 55. 200-207 (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] "Effect of the oral administration of sepinostat mesilate on ceralein induced acute pancreatitis in rats." Arzoneimitle Forshung.Drug Research. 45. 1082-1086 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] "Intracellular Ca ^<2+> dynamics and in vitro secretory response in acute pancreatitis induced by a cloline-deficient ethianine supplemented diet in mice." Digestion. 56. 502-505 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] "Increascd pancreatic netallothionein and glutathion levels : protecting against cerulein and tearocholati induced acute pancreatitis in rats." pancreas.(in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohshio G. et al.: "Pancreatic exocrme secretin in short-term pancreatic duct obstructim induced acute pancreatitis" Digestim. 55. 200-207 (1994)
• [Publications] Wang ZH et al.: "Effect of the oral administration of sepinostat mesilate on cerulein induced acute pancreatitis in rats" Arzneimttel Forschung Drug Research. 45. 1082-1086 (1995)
• [Publications] Okada N et al.: "Intracellular Ca^<2+> dynamics and in uitro secretory responsein acute pancreatitis induced by a choline-deficieut ethinoneine supplemeuted diet in mice" Digestin. 56. 502-508 (1995)
• [Publications] Wang ZH et al.: "Increased pancreatic metallothionein and glutathion levels: protecting against cerulein-and taurocholate-induced acute pancreatitis in rats." Pancreas. (in press).
• [Publications] Ohshio G.: "Pancreatic exocrinl secretion in short-term pancreatic duct obstrwction lnduud acute pancreotitis in rats:An in vivo and in vitro study." Digestion. 55. 200-207 (1994)
• [Publications] Yotsumoto F,Ohshio G.et al.: "Platelet-activating factor inuolvement in the aggravrtion of acute pancreatiti in rabbits." Digestion. 55. 260-267 (1994)
• [Publications] Wang,Z.H.Ohshio G et al.: "Effect of the oral administrntion of sepinostat mesilatl on cerulein induced acute pancreatitis in rats." Arzneimitiel Forschung Dvng Research. (in press).
• [Publications] Inamura T.Ohshio G et al.: "Quantitative analysis of collagen and collagen subtype-I, subtype-III, and subtype-V in human pancreatic-cancer, tumor-associated chronic-pancreatitis, and alcoholic chronic-pancreatitis" Pancreas.(in press).
• [Publications] Inokuma T.Ohshio G et al.: "Evaluation of pancreatic tumers with positron emission tomography and F-18 Fluorodeoxyglucose - comparison with CT and US" Radiology. (in press).
• [Publications] Okada N.Ohshio G et al.: "Intracellnlar Ca^<2+> dynamics and in vitro secretory response in acute pancreatitis induced by a choline-deficlent ethlonine-supplimentcd dietinmiu" Digestion. (in press).