| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
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| Research Abstract |
Hepatopancreatoduodenectomy (HPD) still has many problems and its operative mortality is unfortunately very high. To evaluate the effects of hepatopancreatectomy on the regenerative process of the liver, serum protein changes and hepatic protein synthesis (HPS) as well as Bromodeoxyuridine (BrdU) labeling index were measured. Sprague-Dawley rats were divided into the following groups, according to the types of organ resection : a simple laparotomy was performed in the sham group ; 68% of the liver was excised in the Hx group ; 45% of the pancreas was excised in the Px group ; 45% of the pancreas and 68% of the liver were excised simultaneously in the HPx group. Serum total protein and albumin levels were significantly lower in the HPx group compared to other three groups on postoperative day (POD) 3 (p<0.05). HPS was measured by ^<14>C-leucine incorporation into the hepatic protein fraction. HPS markedly increased in the Hx and HPx groups, both involving hepatectomy. In the Hx group, HPS was significantly higher with a peak on POD 2 compared to the HPx group (p<0.05). On the other hand, in the HPx group, HPS was significantly higher with a peak on POD 3 compared to the HPx group (p<0.05). BrdU labeling index, as a marker of DNA synthesis in regenerating liver, was significantly suppressed in the HPx group on POD 1 compared to the Hx group (p<0.05). It is summarized that a hepatopancreatectomy suppresses DNA synthesis which causes a delay in the increase of protein synthesis in the regenerating liver and further results in the decrease of the serum protein level more than a hepatectomy alone.
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