Protective effect of ADF in reperfusion injury after canine by transplantation
| Project/Area Number |
06671340
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YOKOMISE Hiroyasu Kyoto University Chest Disease Research Institute Thoracic Surgery, Assistant Prof., 胸部疾患研究所, 助手 (80231728)
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| Co-Investigator(Kenkyū-buntansha) |
INUI Kenji Kyoto University Chest Disease Research Institute Thoracic Surgery, Associate, 講師 (10193567)
WADA Hiromi Kyoto University Chest Disease Research Institute Thoracic Surgery, Associate Pr, 助教授 (90167205)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Transplantation Lung / Ischemia reperfusion injury / ADF / human Thioredoxin / 虚血再潅流障害 |
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| Research Abstract |
Human thioredoxin, which was previously recognized as adult T-cell leukemia-derived factor, has many physiologic activities, one of which is a radical scavenger effect. Its ability to reduce reperfusion injury was assessed in vivo in a canine lung transplantation model. In 19 dogs, left lung allotransplantation was performed after 100 minutes of warm ischemia. The function of the transplanted lung was assessed after clamping of the contralateral pulmonary artery. In the human thioredoxin group (n=6), human thioredoxin 30 mg/kg was given to the recipients during reperfusion. In the N-acetylcysteine group (n=5), N-acetylcysteine 150 mg/kg, known as a radical scavenger, was given in the same manner. In both groups, arterial oxygen tension was significantly higher than in the control group (n=8). In the human thioredoxin group, peak inspiratory pressure was significantly lower than in the control group. Macroscopic and microscopic examinations showed an almost normal appearance of the lung tissues in the human thioredoxin and N-acetylcysteine groups, in contrast to the abnormal findings in the control group. Thus it would appear that human thioredoxin has a protective effect on transplanted lungs, as does N-acetylcysteine, and that its action may be a radical scavenger effect.
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Report
(3 results)
Research Products
(13 results)
