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Experimental study of gene therapy for malignant brain tumor

Research Project

Project/Area Number 06671392
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KURISU Kaoru  Hiroshima University School of Medicine Professor, 医学部, 教授 (70201473)

Co-Investigator(Kenkyū-buntansha) UOZUMI Tooru  Hiroshima University School of Medicine Professor, 医学部, 教授 (20028426)
SUGIYAMA Kazuhiko  Hiroshima University Medical Hospital Assistant Professor, 医学部・附属病院, 講師 (30243554)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsMalignant brain tumor / Gene therapy / Cytokine
Research Abstract

Fundamental experiments of the gene therapy for malignant brain tumor were performed by transfection of cytokine genes, cell regulation genes and cell structure genes. As for cytokine genes we confirmed antitumor effect to the malignant glioma cells both in vitro and in vivo by transfer of TNF-alpha gene or INF-beta gene so far. In addition of these results, We transferd of G-CSF gene into human glioma cells and then transplanted them into subcutaneous space of nude mouse. As a result of this experiment, nude mice that were transplanted G-SCF gene transfected glioma cells did not from solid tumor and histological evaluation of the G-CSF gene transfected tumor revealed intense invasion of granulocytes into the tumor. We revealed delation or mutation of cell regulation genes such as p21 and p16 in human glioma cells. So transfection these cell regulation genes into human glioma cells was performed. As aresult of transfection, growth suppression of human glioma cell was revealed. This result leads to the possibility that transfer of cell regulation genes may be the candidate of the gene therapy for the malignant glioma. GFAP is one of the cell structure component and the expression of GFAP seems to relate to cell differentiation. In this study, we transfected GFAP gene into medulloblastoma cells, resulting in suppressed cell growth and increased sensitivity to anticancer drugs. These results indicate that chemotherapy to medulloblastoma may be much more effective by transferring GFAP gene into tumor cells.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 松岡隆: "ラット9L gliosarcomaに対するMacrophage Colony-stimulating factor(M-CSF)の抗腫瘍効果の検討" 神経免疫研究. 7. 305-308 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Maeda: "Combined antitumor effects of TNF and G-CSF on a human medulloblastoma xenograft line" Journal of Neuro-Oncology. 21. 203-213 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunyu Harada: "Growth inhibition of sub cutaneously transplanted human glioma by transfection-induced tumor necrosis factor-α and augmentation of the effect by γ-interferon" Journal of Neuro-Oncology. 22. 221-225 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Matsuoka: "Antitumor effects of human recombinant macrophage Colony-stimulating factor against rat brain tumors" Biotherapy. 8. 51-62 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunyu Harada: "Growth Inhibition of Intracerebral Rat Glioma by Transfection-Induced Human・Interferon-β" Journal of Surgical Oncology. 59. 105-109 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 貞友隆: "G-CSF遺伝子導入による脳腫瘍細胞の増殖に及ぼす影響" 神経免疫研究. 8. 267-271 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 貞友隆: "アンチセンスベクターを用いたグリオーマにおけるテネイシンの機能解析" 脳神経外科速報. 5. 681-685 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunyu Harada: "Analysis of sui-1 Gene Function in Human Malignant Gliomas" Brain Tumor Reserch and Therapy. 435-440 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Sadatomo: "New Approach for the Treatment of Medulloblastoma by Transfection with Glial Fibrillary Acidic protein Gene" Journal of Surgical Oncology. (in print). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Matsuoka: "Antitumor Effect of M-CSF on gliomas Subcutaneous Transplanted in Rats" Neuroimmunological Research. Vol.7. 305-308 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitosi Maeda: "Combined antitumor effects of TNF and G-CSF on a human medulloblastoma xenograft line" Journal of Neuro-Oncology. 21. 203-213 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Konyu Harada: "Growth inhibition of subcutaneously transplanted human glioma by transfection-induced fumer necrosis factor-alpha and augmontation of the effect by alpha-interferon" Journal of Neuro-Oncology. 22. 221-225 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Matsuoka: "Antitumor effects of human recombinant macrophage colony-stimulating factor against rat brain tumor" Biotherapy. 8. 51-62 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunyu Harada: "Growth Inhibition of Intracerebral Rat Glioma by Tramsfection-Induced Human Interferon-beta" Journal of Surgical Oncology. 59. 105-109 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Sadatomo: "Transfer of G-CSF gene into Human Glioma Cells : Effect on the pioliferation of Glioma Cells in Nude Mice" Neuroimmunological Research. Vol.8. 267-271 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Sadatomo: "Analysis of Human Tenascin in Glioma Cells Using Antisense Vector" Noshinkeigekasokuho. 5. 681-685 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takashi Sadatomo: "New Approach for the Treatment of Medulloblastoma by Transfection with Glial Fibrillary Acidic Protein Gene" Journal of Surgical Oncology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunyu Harada: "Analysis of sdi-1 Gene Functions in Human Malignant Gliomas" Brain Tumor Researchand Therapy. 435-440 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kunyu Harada: "Growth Inhibition of Intracerebral Rat Glioma by Transfection-Induced Human Interferon-β" Journal of Surgical Oncology. 59. 105-109 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 貞友 隆: "G-CSF遺伝子導入による脳腫瘍細胞の増殖に及ぼす影響" 神経免疫研究. 8. 267-271 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 貞友 隆: "アンチセンスベクターを用いたグリオーマにおけるテネイシンの機能を解析" 脳神経外科速報. 5. 681-685 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kunyu Harada: "Analysis of sdi-1 Gene Function in Human Malignant Gliomas" Brain Tumor Reserch and Therapy. 435-440 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takashi Sadatomo: "New Approach for the Treatment of Medulloblastoma by transfection with Glial Fibrillary Acidic Protein Gene" Journal of Surgical Oncology. (in print). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kunyu Harada: "Growth inhibition of subintaneously transplanted human glioma by traisfection-induced tumor necrosis factor-αand augmentation of the effect by α-interferon." Journal of Neuro-Oncology. (in print). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kunyu Harada: "Analysis of sdi-1 gene functions in human malignant glioma" Brain Tumor Resarch and Therapy. (in print). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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