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The of the neurotrophic factor to the ischemic brain (in the aspect of the spatial learning ability)

Research Project

Project/Area Number 06671396
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionEhime University School of Medicine

Principal Investigator

KUMON Yoshiaki  Ehime University, School of Medicine Assistant Professor, 医学部, 助教授 (80127894)

Co-Investigator(Kenkyū-buntansha) OHUE Shiroh  Ehime University, School of Medicine Lecture, 医学部, 助手 (70213626)
KOHNO Kanehisa  Ehime University, School of Medicine Lecture, 医学部, 講師 (60145076)
善家 喜一郎  愛媛大学, 医学部, 助手 (60236130)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsfocal cerebral ischemia / neurotrophic factor / Morris water maze test / ciliary neurotrophic factor / thalamus / intraventricular infusion / 実験的脳虚血 / モリス水迷路学習 / マイネルト核 / コリンアセチルトランスフェラーゼ / 塩基好性線維芽細胞成長因子 / 脳室内持続注入
Research Abstract

The effect of repeated Morris water maze tests on the ability of place navigation was examined in rats with permanent unilateral occlusion of the middle cerebral artery, in relation to choline acetyltransferase (ChAT) immunoreactivity in the basal magnocellular nucleus of Meynert (NBM). MCA-occluded rats subjected to repeated water maze tests exhibited only transient disability of place-navigation. Despite the time dependent recovery of place-navigation activity in MCA-occluded rats, ChAT neurons in the NBM,which are considered to be involved in spatial learning ability, were fewer on the ischemic side than the non-ischemic side throughout the examination period. These findings suggest that early water maze training facilitates the functional restoration of MCA-occluded rats, and the recovery of place-navigation activity in the ischemic rats cannot necessarily be explained by a reduction in the ischemic injury of ChAT neurons in the NBM.
Ciliary neurotrophic factor (CNTF) has been shown to exhibit potent neurotrophic activity on peripheral and central neurons in vitro and in vivo. We infused CNTF continuously for 4 weeks into the lateral ventricle, starting just after permanent occlusion of the left middle cerebral artery (MCA) of stroke-prone spontaneous hypertensive rats. CNTF infusion prevented the occurrence of ischemia-induced learning disability in a dose-dependent manner in rats subjected to the Morris water maze task. Subsequent histological examinations showed that cortical infarction and retrograde degeneration of the ipsilateral thalamic neurons in ischerwc rats infused with CNTF were significantly less severe than those in ischemic rats infused with vehicle alone. These findings suggest that postischemic CNTF treatment prevents the occurrence of spatial learning disability in rats with permanent MCA occlusion, possibly by reducing neuronal damage within the cerebral cortex and secondary retrograde degeneration of the thalamus.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 久門 良明: "Spatial leaming disability in rats with focal cerebral ischemia recovers by repeating Morris water mazetests" Biomedical Research. 16. 423-431 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久門 良明: "Ciliary neurotrophic factor attenuates spatial cognition impairment, cortical infarction and thalamic degeneration in spontaneously hypertensive rats with focal cerebral ischemia" Neuroscience Letters. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiaki Komon, Saburo Sakaki, Toshihoro Ueda, et al: "Spitial learning disability in rats with focal cerebral ischemia recovers by repeating Morris water maze tests." BIOMEDICAL RESEARCH. 16. 423-431 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yoshiaki Kumon, Saburo Sakaki, Hideaki Watanabe, et al: "Ciliary neurotrophic factor attenuates spatial cognition impairment, cortical nfarction and thalamic degeneration in spontaneously hypertensive rats with focal cerebral ischemia." NEUROSCIENCE LETTERS. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久門 良明: "Spatial learning disability in rats with focal cerebral ischemia recovers by repeating Morris water maze tests" Biomedical Research. 16. 423-431 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久門 良明: "Ciliary neurotrophic factor attenuates spatial cognition impairment,cortical infarction and thalamic degeneration in spontaneously hypertensive rats with focal cerebral ischemia." Neuroscience Letters. (in press).

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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