• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of cerebral vasospasm underlying intracellular signal transduction and contractile protein of the cerebral vascular smooth muscle cells.

Research Project

Project/Area Number 06671416
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionIWATE MEDICAL UNIVERSITY

Principal Investigator

DOI Mamoru  Iwate Medical University, Assistant professor, 医学部, 講師 (60197994)

Co-Investigator(Kenkyū-buntansha) SATO Makoto  Iwate Medical University, Professor, 医学部, 教授 (80048248)
OGAWA Akira  Iwate Medical University, Professor, 医学部, 教授 (10204067)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordscalponin / contraction / smooth muscle / subarachnoid hemorrhage / vasospasm
Research Abstract

Calponin, an actin-and tropomyosin-binding protein, has been characterized as an inhibitory factor in the smooth-muscle actomyosin activity. The level of calponin was determined in canine basilar arteries in a double-hemorrhage model. Thirty dogs were assigned to three groups : day 0 group, control ; day 2 group, dogs sacrificed 2 days after cisternal injection of blood ; and day 7 group, dogs given double cisternal injections of blood and sacrificed 7 days after the first injection. Constriction of the basilar artery was confirmed by arterial angiography. Portions of the affected arteries or the corresponding region in control animals were solubilized for sodium dodecylsulfate-polyacrylamide gel electrophoresis and Western blotting. A major band corresponding to calponin was seen at 34 kD in the basilar artery extracts using chicken gizzard polyclonal antibodies. The densitometer values of the band on Coomassie blue-stained gels were expressed as percentages of day 0 control values. The signals of day 2 and day 7 samples were 47%<plus-minus>20% and 23%<plus-minus>12%, respectively (mean<plus-minus>standard deviation). The proportions of calponin to actin/tropomyosin in the day 0, day 2 and day 7 groups were 13%<plus-minus>6%, 6%<plus-minus>2%, and 4%<plus-minus>2%, respectively. The reduced expression of calponin may be related to sustained contraction during cerebra

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 土肥守 他: "脳血管攣縮における平滑筋収縮制御蛋白の関与" 脳血管攣縮. 10. 161-165 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 太田浩彰、土肥守、紺野広、佐藤誠、小川彰: "各種脳血管攣縮物質の相互増強効果の定量的解析" 脳血管攣縮. 10. 147-151 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 土肥守 他: "脳血管障害に対するケタスの使用経験" 新薬と臨床. 43. 2093-2105 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 土肥守、小川彰: "ウシ中大脳動脈における各種収縮物質に対するibudilastの弛緩効果の発生機序について" 東北脳血管障害懇話会. 17. 55-59 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sasaki,M.Sato et al: "Depressing effects of protein kinase A and C on thercceplor-induced kiturrent responses in the ganglion cells of aphysis" Nether land Journal of Zoology. 44. 578-587 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 土肥守、小川彰 他: "血小板由来MAP kinase activatorによる脳血管攣縮の発生機序" 脳血管攣縮. 11(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mamoru Doi, Hidetoshi Kasuya, Bryce Weir, David Cook, Akira Ogawa: "Involvement of contractile control proteins in the development of cerebral vasospasm after subarachnoid hemorrhage" Cerebral Vasospasm. (Jpn) 10. 161-165 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M Doi, Y Otawara, N Kubo, A Suzuki, T Hidaka, A Ogawa: "Clinical trial of ibudilast on the patients of cerebral vascular disease" New drugs and clinic. 43(10). 2093-2105 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A Ohta, M Doi, H Konno, M Sato, A Ogawa: "Quantitative analysis of matually augment effects of various spasmogenic substances studies on the bovine cerebral arteries" Cerebral Vasospasm. (Jpn) 10. 147-151 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mamoru Doi, Akira Ogawa: "Mode of cerebral vasodilating action of ibudilast in isolated bovine middle cerebral artery" Tohoku Cerebro-vascular disease conference. 17. 55-59 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K Sasaki, M Matsumoto, K Takashima, R Fujita, S Kawasaki, S Kimura, M Sato: "Depressing effects of protein kinases A&C on the receptor-induced K+-current responses in the ganglion cells of aplysia" Netherland Journal of Zoology. 44 (3-4). 578-587 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mamoru Doi, Koh Tsuiki, Michiyasu Suzuki and Akira Ogawa: "Mechanisms of contraction induced by MAP kinase activator derived from platelet" Cerebral Vasospasm. (Jpn) 11. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mamoru Doi, Hidetoshi Kasuya, Bryce Weir, David Cook, Akira Ogawa: "Reduced expression of calponin in canine basilar artery after subarachnoid hemorrhage" (Submitted to neurosurgery). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 土肥守他: "脳血管攣縮における平滑筋収縮制御蛋白の関与" 脳血管攣縮. 10. 161-165 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 太田浩彰、土肥守、紺野広、佐藤誠、小川彰: "各種脳血管攣縮物質の相互増強効果の定量的解析" 脳血管攣縮. 10. 147-151 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 土肥守、小川彰: "ウシ中大脳動脈における各種収縮物質に対するibukilaseの弛緩効果の発生機序について" 第17回脳血管障害懇話会講演集. 17. 55-59 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 土肥守、小川彰: "血小板由来MAP kinase activatorによる脳血管攣縮の発生機序" 脳血管攣縮. 11(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 土肥 守: "脳血管攣縮における平滑筋収縮制御蛋白の関与" 脳血管攣縮. 10. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 太田浩彰,土肥 守,紺野 広,佐藤 誠,小川 彰: "各首脳血管攣縮物質の相互増強効果の定量的解析" 脳血管攣縮. 10. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 土肥 守,小川 彰: "ウシ中大脳動脈における各種収縮物質に対するibudilaseの弛緩効果の発生機序について"

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi