• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Measurement of Blood Flow Velocity in Cerebral Microvessels by a Fiber-Optic Laser-Doppler Anemometer Microscope

Research Project

Project/Area Number 06671432
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionNational Cardiovasular Center Reseauch Institute

Principal Investigator

SEKI Junji  Natl.Cardiovasc Ctr Res Inst.Dept Biomed Eng, Research Fellow, 生体工学部, 室長 (20163082)

Co-Investigator(Kenkyū-buntansha) TAKAMIZAWA Keiichi  Natl.Cardiovasc Ctr Res Inst.Dept Biomed Eng, Research Fellow, 生体工学部, 室員 (10163312)
MATSUDA Takehisa  Natl.Cardiovasc Ctr Res Inst.Dept Biomed Eng, Head of Dept, 生体工学部, 部長 (60142189)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsBrain Pial Microvessels / Laser-Doppler Anemometer / Pulsatile Flow / Amplitude of Pulsation / Wave Propagation / Propagation Velocity / Vessel Wall Elasticity / Intracranial Pressure / 血流脈波
Research Abstract

This research project was aimed to establish the applicability of our developed fiber-optic laser-Doppler anemometer microscope (FLDAM) to the measurement of red cell velocity in the cerebral pial microvessels and was also aimed to analyze the blood flow dynamics under the increased intracranial pressures.
A closed cranial window was implanted on one side of the parietal region of rats to observe the brain pial microcirculation. The red cell velocity in single pial microvessels with diameter range from 10 to 230 mum was successfully measured by the FLDAM.The red cell velocity in arterioles showed regular pulsation synchronous with the heart beats with amplitude up to 46 % of the mean velocity, while the velocity in venules only showed random fluctuations with small amplitude. The amplitude of velocity pulsation was defined as the half of the difference betweenthe maximum and minimum of the ensemble average velocity. The temporal mean velocity in the arterioles increased with increasing … More diameter. It was 3.5 and 14 mm/s for the arteriole with 10 and 50 mum in diameter, respectively, on an average. The time lag of the velocity in the arteriole relative to the femoral arterial pressure increased with the distance along the vessel from upstream to downstream, which indicates the propagation of flow pulse along the arteriole. The propagation velocity of flow pulse was determined for several pial arterioles. It ranged between 10 and 115 cm/s.
Effects of intracranial pressure (ICP) on the pial microcirculation were studied by changing the internal pressure of the closed cranial window implanted in the rats. The average diameter of venules decreased monotonically from 1.1 to 0.78 as the ICP increased from 0 to 50 mmHg, where the diameter is normalized by the diameter at ICP=5mmHg. On the other hand, the temporal mean velocity in venules increased monotonically from 0.85 to 1.91. As a result, the volume flow rate in venules did not change significantly with ICP.The changes of arterioles with ICP were small compared with those of venules. The amplitude of velocity pulsation in arterioles showed stepwise increase with ICP at a certain value of ICP,ICP_<cr'> which depended on the location of the vessel. ICP_<cr> increased with increasing vessel diameter and decreased from upstream to downstream along an arteriole, suggesting that ICP_<cr> represents the intravascular pressure of the arteriole at a certain phase of pulsation. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Seki, J., Sasaki, Y., Oyama, T. & Yamamoto, J.: "Blood Flow in Pial Microvessels in Rats under Open and Closed Cranial Window Preparations" Progress in Microcirculation Research. 171-174 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 畑中美博,関淳二,松田武久: "体外循環時の抹消循環病態モデル:ラット腸間膜微小血管における白血球の転がりと粘着に及ぼすアナフィラトキシンC5aの影響" 人工臓器. 23. 1018-1024 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J.: "Measurements of Propagation Velocity of Flow Pulse in Microvessles by a Two-Point Laser-Doppler Anemometer Microscope" Microcirculation Annual 1994. 10. 199-200 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 関 淳二: "2点LDA顕微鏡による微小血管ネットワークにおける脈波伝播過程の解析" 流れの計測. 12. 37-42 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J., Sasaki, Y., Oyama, T. & Yamamoto, J.: "Effects of Increased Intracranial Pressure on the Blood Flow Pulsation in Rat Pial Microvessels" Microcirculation Annual 1995. 11. 121-122 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasaki, Y., Seki, J., Giddings JC, Yamamoto J.: "Effects of NO-Donors on Thrombus Formation and Microcirculation in Cerebral Vessels of the Rat" Thrombosis and Haemostasis. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J., Sasaki, Y., Oyama, T., Yamamoto, J.: "Blood Flow in Pial Microvessels in Rats under Open and Closed Clanial Window Preparations" in "Progress in Microcirculation Research", ed. by H.Niimi, M.Oda, T.Sawada and R-J.Xiu, Pergamon. 171-174 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hatanaka, Y., Seki, J., Matsuda, T.: "Microcirculatory Prediseased State Model Induced by Extracorporeal Circulation : C5a-induced Neutrophil Adhesion on Venular Endothelium" Jpn.J.Artif.Organs. 23 (4). 1018-1024 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J.: "Propagation of Flow Pulse in Microvessels" J.Jpn.Soc.Biorheology. 8 (4). 209-210 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J.: "Measurements of Propagation Velocity in Microvessels by a Two-point Laser-Doppler Anemometer Microscope" Microcirculation Annual 1994,10, Nihon lgakukan, Tokyo. 199-200 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J., Sasaki, Y., Oyama, T., Yamamoto, J.: "Effects of intracranial Pressure on the Brain Pial Microcirculation in the Rats" Jpn.J.Med.Electron.Biol.Eng.32 (Suppl.). 369 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J., Sasaki, Y., Oyama, T., Yamamoto, J.: "Effects of Increased Intracranial Pressure on the Blood Flow Pulsation in Rat Pial Microvessels" Microcirculation Annual 1995,11, Nihon Igakukan, Tokyo. 121-122 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J., Sasaki, Y., Oyama, T., Yamamoto, J.: "Effects of Intracranial Pressure on the Blood Flow Pulsation in the Rat Pial Microvessels" Proc. 2nd Asian Cong. of Microcirc., Beijing. 17. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sasaki, Y., Seki, J., Giddings, J.C., Yamamoto, J.: "Effects of NO-Donors on Thrombus Formation and Microcirculation in Cerebral Vessels of the Rat" Thrombosis and Haemostasis. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seki, J., Sasaki, Y., Oyama, T. & Yamamoto, J.: "Effects of Increased Intracranial Pressure on the Blood Flow Pulsation in Rat Pial Microvessels" Microcirculation Annual 1995. 11. 121-122 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 関 淳二: "2点LDA顕微鏡による微小血管ネットワークにおける脈波伝播過程の解析" 流れの計測. 12. 37-42 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 関 淳二: "2点レーザ流速計顕微鏡による微小血管ネットワークにおける脈波伝播過程の解析" 医用電子と生体工学. 33. 289- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seki, J., Sasaki, Y., Oyama, T. & Yamamoto, J.: "Effects of Intracranial Pressure on the Blood Flow Pulsation in the Rat Pial Microvessels" Proc. of 2nd Asian Cong. of Microcirc.17- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sasaki, Y., Seki, J., Giddings JC, Yamamoto J.: "Effects of NO-Donors on Thrombus Formation and Microcirculation in Cerebral Vessels of the Rat" Thrombosis and Haemostasis. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oyama T, Sasaki Y, Giddings JC, Seki J. & Yamamoto J.: "Cerebral Prothrombotic State in Spontaneously Hypertensive Rats of Different Breeds" Haemostasis. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seki,J.,Sasaki,Y.,Oyama,T.& Yamamoto,J.: "Blood Flow in Pial Microvessels in Rats under Open and Closed Cranial Window Preparations" Progress in Microcirculation Research. 171-174 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 関 淳二: "微小血管における血流の拍動、脈波の伝播" 日本バイオレオロジー学会誌. 8. 209-210 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 畑中美博,関淳二,松田武久: "体外循環時の抹消循環病態モデル:ラット腸間膜微小血管における白血球の転がりと粘着に及ぼすアナフィラトキシンC5aの影響" 人工臓器. 23. 1018-1024 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Seki,J.: "Measurements of Propagation Velocity of Flow Pulse in Microvessels by a Two-Point Laser-Doppler Anemometer Microscope" Microcirculation Annual 1994. 10. 199-200 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Seki,J.,Sasaki,Y.,Oyama,T.& Yamamoto,J.: "Measurements of Brain Pial Microcirculation in Rats under High Intracranial Pressures with a Fiber-Optic Iaser-Doppler Anemometer Microscope" Pathophysiolgy. 1. 394- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Oyama T,Sasaki,Y,Giddings JC,Seki J.& Yamamoto J.: "Cerebral Prothrombotic State in Spontaneously Hypertensive Rats of Different Breeds" Haemostasis. (in press). (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi