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c-fos protein activation and functional analysis in central nervous system after transcranial magnetic stimulation

Research Project

Project/Area Number 06671446
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

KITAGAWA Hideki  Toyama Medical & Pharmaceutical University, Department of Orthopedic Surgery, lecturer, 附属病院, 講師 (70242500)

Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
KeywordsMagnetic stimulation / c-fos protein / motor evoked potential / C-fos遺伝子 / 磁気刺激
Research Abstract

Neural activation and conducting pathways after transcranial magnetic stimulation were studied by c-fos protein analysis and evoked spinal cord and evoked compound muscle action potential (ESCP and ECMAP) analysis. c-fos protein was stained in the nucleus of pyramidal and extrapyramidal tracts, which was observed in the nucleus over the entire cerebral cortex. Therefore, transcranial magnetic stimulation was supposed to be spread not only the motor cortex but also the entire cerebral cortex.
To obtain the optimal conditions of the transcranial magnetic stimulation in cats. ECMAP and ESCP in response to transcranial magnetic stimulation, and magnetic and electric field were analyzed in cats with and without permalloy shield.
Ten adult cats were anesthetized with ketamine and subjected to transcranial magnetic stimulation with an 8 figure magnetic coil under a shield of permalloy that had variable windows open to the cortical motor area.Firstly, ECMAP and ESCP were recorded with an 8 figur … More e coil under no permalloy shield or under various windows of permalloy shield. Secondly, because magnetic field shield, or permalloy, altered the size and homogeneity of the magnetic field, magnetic and electric fields under magnetic coil were studied with and without permalloy.
The maximum amplitude of ECMAP was recorded when the coil was placed on-catユs cranium at an angle of 90 degrees with and without a permalloy shield with a 5x5 cm window. Thresholds of ECMAP and ESCP were lower under a permalloy shield with a 5x5 cm window than without a permalloy. Strength of magnetic and electric field were highest at the brain stem area. Thus, large induced electric field directed caudally elicited ECMAP and ESCP effectively at a coil angle of 90 degrees with a 5x5 cm window permalloy.
Transcranial magnetic stimulation revealed diffuse c-fos protein activity of the nucleus in the motor cortex, diffuse cerebral cortex, and brain stem. Modified electric field conceivably stimulated centrifugal pathways at the vicinity of the brain atem and entire cerebral cortex. Less

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Kitagawa H.: "Effects of coil orientation and magnetic shield to transcranial magnetic stimulation in cats." Muscle and nerve. (予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 北川秀機: "経頭蓋磁気刺激下の中枢神経c-fos蛋白活性。" 脳波と筋電図. (予定). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中藤真一、高野治雄、北川秀機、中村 宏: "パ-マロイによるネコ経頭蓋磁気刺激のいき値変化." 脊髄電気診断学. 17. 33-36 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideki Kitagawa, MD,Shinichi Nakatoh, MD,Yoshiharu Kawaguchi, MD,Hiroshi Nakamura, MD,and Haruo Tsuji, MD: "Effects of coil orientation and magnetic field shield on transcranial magnetic stimulation in cats." submitted in Muscle and nerve. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideki Kitagawa, MD.: "c-fos protein activation in central nervous system after transcranial magnetic stimulation in cats." in preparation for submission to EEG and EMG,(in Japanese). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinichi Nakatoh, M.D., Haruo Takano, M.D., Hideki Kitagawa, MD., Hiroshi Nakanura, M.D.: "Threshold changes by Permally after transcranial magnetic stimulation in cats." J.of electrodiagnosis of the spinal cord (in Japanese). 17. 33-36 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitagawa H.: "Effects of coil orientation and magnetic shield to transcranial magnetic stimulation in cats." Muscle and nerve. (予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] 北川秀機: "経頭蓋磁気刺激下の中枢神経c-fos蛋白活性。" 脳波と筋電図. (予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 中藤真一、高野治雄、北川秀機、中村宏: "パ-マロイによるネコ経頭蓋磁気刺激のいき値変化." 脊髄電気診断学. 17. 33-36 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北川秀機: "パ-マロイによるネコ経頭蓋磁気刺激のいき値変化" 脊髄電気診断学. 17. 33-36 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hideki Kitagawa: "Magnetic evoked compound muscle action potential neuromonitoring in spine surgery" Spine. (in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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