| Project/Area Number |
06671462
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
IWAMOTO Yukihide KYUSHU UNIVERSITY,MEDICAL DEPT., ASSOCIATE PROFESSOR, 医学部, 助教授 (00213322)
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| Co-Investigator(Kenkyū-buntansha) |
泉 敏弘 九州大学, 医学部, 助手 (90253426)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | MALIGNANT TUMOR / BONE AND SOFT TISSUE / METASTASIS / BASEMENT MEMBRANES / INVASION / 異性腫瘍 / 規定膜 |
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| Research Abstract |
Tumor invasion of the basement membranes is a crucial step in the complex multistage process which leads to the formation of metastasis. Tumor cells cross the basement membranes as they initially invade vascular beds during dissemination, and then they penetrate their target tissue. The treatment of human fibrosarcoma cells HT1080 with cAMP analogues and chorela toxin that increase the intra-cellular cAMP concentrations induces the expression of Timp-1 and Timp-2 both at the mRNA and the protein levels. This induction of Timps correlated well with type IV collagenolytic activity, invasiveness and metastatic potential of HT1080 cells.
We developed a quantitative assay for assessing in vivo tumor angiogenesis using basement membrane extracts (Matrigel). Nude mice are injected s.c. with liquid Matrigel mixed with HT1080 cells. Since Matrigel forms a solid gel rapidly at body temperature, the gel containing tumor cells can be removed immediately, and the processed for histological studies. Tumor angiogenesis was monitored quantitatively by measuring both the number and total area of neovessels present in the gels using an image analyzer.
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