| Project/Area Number |
06671463
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | NAGASAKI UNIVERSITY |
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Principal Investigator |
FUJII Tohru Nagasaki Univ.Sch.of Med.Dept.of Plastic & Reconstructive Surgery Professor, 医学部, 教授 (60136661)
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| Co-Investigator(Kenkyū-buntansha) |
NAMBA Hiroyuki Nagasaki Univ.Sch.of Med.Dept.of Cell Physiology Associate Professor, 医学部・原研発症予防部門, 助教授 (80237635)
YAMASHITA Shunichi Nagasaki Univ.Sch.of Med.Dept.of Cell Physiology Professor, 医学部・原研発症予防部門, 教授 (30200679)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Parathyroid hormone related protein / Transforming growth factor beta / Anti-sense / S-oligo-DNA / Gene therapy / Cleft palate |
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| Research Abstract |
Normal palate develops through the vertical growth, the bilateral elevation and fusion. We investigated expression of TGT beta and PTHrP in this fine process of the palate development using immunohistochemistry and in situ hybridization. Expression of both factors were recognized in the epithelium of palatal shelves and the mesenchymal condensation for bone. Concerning administration of Vitamine A induce cleft palate in pregnant rodents, reduction of PTHrP expression in embryonic facial tissue and decrease of DNA synthesis were also recognized from Vitamine A administration. However we could not show a good result of intra-amniotic administration of anti-sense S-oligo DNA for PTHrP.Although FITC-oligo was confirmed to be taken to the embryo, no remarkable change was observed. From now on, we would use organ culture, and attempt to elucidate the cleft-palate etiology.
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