| Project/Area Number |
06671471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara medical university |
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Principal Investigator |
TAKAKURA Yoshinori MEDICAL,NARA MEDICAL UNIVERSITY,ASSISTANT PROF., 医学部, 助教授 (40094578)
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| Co-Investigator(Kenkyū-buntansha) |
DOHI Yoshiko MEDICAL,NARA MEDICAL UNIVERSITY,ASSISTANT PROF., 医学部, 助教授 (50155628)
MII Yoshio MEDICAL,NARA MEDICAL UNIVERSITY,ASSISTANT PROF., 医学部, 助教授 (70145845)
OHGUSHI Hajime MEDICAL,NARA MEDICAL UNIVERSITY,LECTURER, 医学部, 講師 (80213669)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | hydroxyapatite / porous alumina ceramics / osteogenesis / bone marrow cell / 生化学的研究 |
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| Research Abstract |
Alumina ceramic (Al) has good biocompatibility and high mechanical strength and has been used for total joint replacements. However, it does not bond to bone tissue resulting in complications such as loosening. Hydroxyapatite ceramic (HA) is a bioactive material having bone-bonding properties, and so we developed HA coated porous alumina ceramic (HA/Al) having bioactive properties. In this study, we compared the osteogenic response in porous Al and HA/Al. The osteogenic stimulus was derived from bone marrow cells. Both ceramics alone and combined with rat bone marrow cells were implanted subcutaneously in the back of syngeneic rats and harvested 4 and 8 weeks after implantation. The ceramics (Al and HA/Al) without marrow did not show any bone formation, however, when combined with marrow cells, they showed bone formation. The bone formation in HA/Al began directly on the ceramic surface, whereas the bone formation in Al began away from the ceramic surface and fibrous tissue interposition was seen between the de novo bone and its surface. Alkaline phosphatase (ALP) activity and bone specific bone gla protein (BGP) content in HA/Al with marrow were both about 3 times higher than in Al with marrow. These results indicate that HA/Al has excellent osteoconductive properties and that a composite of marrow and HA/Al is a clinically applicable osteogenic biomaterial.
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