| Project/Area Number |
06671482
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Juntendo University School of Medicine, Deparyment of Orthopedic surgery |
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Principal Investigator |
YAMAUCHI Yasuo Juntendo University School of Medicine, Professor, 医学部, 教授 (00052994)
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| Co-Investigator(Kenkyū-buntansha) |
YONEZAWA Ikuho Juntendo University School of Medicine,, 医学部・大学院
奥村 康 順天堂大学, 医学部, 教授 (50009700)
広瀬 友彦 順天堂大学, 医学部, 助手 (20181208)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | integrin / chondrocyte / extracellular matrix / フィブロネクチン / 接着分子 / 機械的ストレス / 分子生物学 / マトリックスメタロプロテアーゼ / サイトカイン / 接着因子 |
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| Research Abstract |
Adhesion molecules of the integrin family, including very late activation antigens (VLA), have been implicated in various cellular functions. In this study, we investigated the contribution of integrin-mediated interaction with ECM proteins to the cytokine gene expression and the response to the shear stress in human chondrocytes
Human articular chondrocytes expressed VLA-1, -2, -3 and -5 on the cell surface, and could adhere to various ECM proteins, especially to fibronectin (FN). Furthermore, the production of GM-CSF and IL.-6 was potently induced by culturing chondrocytes on immobilized FN.This stimulative effect of FN was completely inhibited by an anti-integrin alpha 5 chain mAb, as well as by anti-integrin beta 1 chain mAbs.
Application of shear stress to articular chondrocytes resulted in the up regulation of IL-6 ICAM-1 mRNA, and down regulation of VCAM-l.
These results indicate an important role of the adhesion molecule in regulating inflammatory cytokine production and response to the mechanical stress by human articular chondrocytes.
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