| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
To determine the healing potential and healing process of torn rotator cuff tendons, in situ hybridization was used to localize cells containing procollagen alpha 1 type l and type III messenger RNA (mRNA). Materials and Methods
For determining the specificity and sensitivity of probes, Achilles tendons including skin were taken from four 20-day fetal chicken. Under intravenous anesthesis, complete-thickness and incomplete-thickness tears, which were bursal-side and joint-side tears, were made by cutting the tendons of profund pectoralis of four mature female chicken. The opposite shoulders were used as comparison. They were sacrificed 1, 2 and 4weeks follo wing operations. The specimens were fixed in 10% buffer ed formalin and embedded in paraffin.
Two probes (C11 and C13) were designed to visualize chicken procollagen alpha 1 type l and III mRNA, respectively. These probes were labeled with digoxigenin and used as an in situ marker. As C13 probe did not detect signals of procollagen alpha 1 type III mRNA clearly, this study was dons only by using C11 probe for detecting procollagen alpha 1 type l mRNA.
The labeled cells were mainly composed of the tenocytes and undifferentiated mesenchymal cells. In complete-thickness tears, the number of labeled cells at the proximal tendon stumps of specimens was more abundant at 2 weeks than that at 4 weeks follo wing injuries. The labeled cells at the margins of the tears were detected in the bursal-side and joint-side tears.
In the near future, the quantification of mRNA of procollagens should be performed for comparison and design of new probe for procollagen alpha 1 type III mRNA will be needed.
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